婴幼期大自然声音组合干预对大鼠成年后焦虑样行为的影响

doi:10.13481/j.1671-587X.20210409

Abstract

Abstract: Objective

To clarify the effect of combined natural sound intervention during infancy on the anxiety-like behaviors in the adult rats by testing the offspring male SD rats’ anxiety-like behaviors.

Methods

Six SD rats with 14－15 d of gestation were randomly divided into standard noise control group （NN group），Mozart K448 group （MK group） and combination of natural sound group （NS group）. The rats in NN group were not given any special sound stimulation，the rats in MK group were given 2 h of MK sound stimulation at a volume of 50－70 dB per day for 30 d，and the rats in NS group were given a combination of natural sounds at a volume of 65－75 dB for 2 h a day for 30 d. Five weeks after the end of stimulation， 11 male rats with the similar body weight in NN group and in NS group， and 8 rats in MK group were selected for open field experiment，light-dark transition test and elevated plus-maze test.The open field experiment was used to analyze the total movement distance， rearing times， the number of entering the central area， and the time of entering the central area. The percentage of time staying in light box and the number of transition between light and dark boxes and the total movement distance of rats were analyzed in the light-dark transition test. The elevated plus-maze test was used to analyze the percentage of time in open arms， the number of open arms entries and the distance of open arms.

Results

In the open field experiment， there were no statistically significant differences among three groups in the total movement distance， rearing times， the time of entering the central area， and the number of entering the central area （P>0.05）. The results of light-dark transition test showed that the time in the light box of the rats in NS group was significantly longer than that in NN group （P<0.05）； the number of transition between light and dark boxes of the rats in NS group was also significantly increased （P<0.05 or P<0.01）. The results of elevated plus-maze test also showed that the percentage of time in open-arms of the rats in NS group was significantly longer than that in NN group （P<0.05）.

Conclusion

Intervention of natural sounds during infancy can reduce the anxiety-like behaviors in the mature male SD rats.

Key words: natural environment, natural sounds, music trerapy, anxiety, rats，SD

CLC Number:

R749.059

Qian WANG,Siyue ZHUANG,Guilong WU,Shengtian LI. Effect of combination of natural sounds during infancy on anxiety-like behaviors in adult rats[J].Journal of Jilin University(Medicine Edition), 2021, 47(4): 882-887.

Figures/Tables 6

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Tab.1

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References 25

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Group	n	Total distance(l/m)	Number of rearing	Number of entering central area	Time of entering central area(t/s)
NN	11	13.52±0.90	39.27±3.88	14.18±2.61	24.34±4.12
MK	8	12.11±0.93	34.38±3.49	16.63±3.72	25.41±5.60
NS	11	11.50±1.40	32.63±4.98	8.82±2.12	15.75±4.70

Group	n	Percentage of time in light box(η/%)	Number of transition	Total distance(l/m)
NN	11	32.28±3.46	15.73±1.41	7.35±1.29
MK	8	34.38±1.91	17.88±1.73	6.43±0.33
NS	11	41.38±2.53^*	26.27±2.17^**△	9.16±0.99^*△

Group	n	Percentage of time in open arms(η/%)	Number of open arms entries	Distance of open arms(l/m)
NN	11	11.99±3.94	4.73±1.07	0.83±0.25
MK	8	23.52±6.85	6.28±1.33	1.28±0.39
NS	11	25.06±2.45^*	4.82±0.46	1.24±0.17

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Effect of combination of natural sounds during infancy on anxiety-like behaviors in adult rats

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