Abstract:

Objective To investigate the effects of up-regulation of protein phosphatase Mg²⁺/Mn²⁺-dependent 1F （PPM1F） on the proliferation and migration of nasopharyngeal carcinoma HONE-1 cells， and to clarify their mechanisms. Methods The nasopharyngeal carcinoma HONE-1 cells were divided into pcDNA3.1 group （transfected with pcDNA3.1 plasmid） and pcDNA3.1-Flag-PPM1F group （transfected with pcDNA3.1-Flag-PPM1F plasmid）. The expression levels of PPM1F and E-cadherin mRNA and proteins in the cells in two groups were detected by real-time fluorescence quantitative PCR（RT-qPCR） and Western blotting methods； the activities of proliferation and clone formation rates of the cells in two groups were detected by CCK-8 method and clone formation assay； the scratch healing rates of the cells in two groups were detected by cell scratch assay，and the numbers of migation cells in two groups were detected by Transwell experiment. Results Compared with pcDNA3.1 group， the expression level of PPM1F mRNA in the cells in pcDNA3.1-Flag-PPM1F group was significantly increased（P<0.01），the expression amount of PPM1F protein was significantly increased，the expression levels of E-cadherin mRNA and protein in the cells were significantly increased（P<0.01），the proliferation activity，the clone formation rate and the scratch healing rate were significantly decreased （P<0.05 or P<0.01）， and the number of migration cells was significantly reduced（P<0.05）. Conclusion Up-regulation of PPM1F expression can inhibit the proliferation and migration of nasopharyngeal carcinoma HONE-1 cells， and its mechanism may be related to intercellular adhesion.

Key words: Protein phosphatase Mg²⁺/Mn²⁺-dependent 1F, Nasopharyngeal neoplasms, Cell proliferation, Cell migration