胎盘表达转录因子1在多囊卵巢综合征大鼠卵巢组织中的表达及其对大鼠卵巢颗粒细胞增殖的影响

doi:10.13481/j.1671-587X.20250503

Abstract

Abstract:

Objective To investigate the expression of placenta expressed transcript 1（Plet1）in ovarian tissue of the letrozole-induced model rats of polycystic ovary syndrome （PCOS） and its effect on the proliferation of rat ovarian granulosa cells，and to clarify the possible mechanism by which Plet1 may contribute to the pathogenesis of PCOS. Methods The ovarian tissue samples from the rats collected in previous studies were used and divided into control and PCOS groups. Real-time fluorescence quantitative PCR （RT-qPCR） and Western blotting methods were used to detect the expression levels of Plet1 mRNA and protein in ovarian tissue of the rat in two groups. Additionally， twenty-four rats underwent vaginal smear cytology were divided into four groups by estrous cycle phase： proestrus，estrus，metestrus and diestrus. RT-qPCR was used to detect the expression level of Plet1 mRNA in ovarian tissue of the rats in various groups，and immunohistochemistry （IHC） method was used to detect the location of Plet1 expression in the rat ovarian tissue in various groups. The rat ovarian granulosa cells were transfected and divided into control group， si-Plet1-rat-266 group， si-Plet1-rat-383 group， and si-Plet1-rat-554 group. Cell counting kit-8（CCK-8）method was used to assess the cell proliferation activities of rat ovarian granulosa cells in various groups， and RT-qPCR method was used to detect the expression levels of cyclin-dependent kinase 6 （CDK6） and P53 mRNA in rat ovarian granulosa cells in various groups. Results The RT-qPCR results revealed that Plet1 mRNA was expressed in the ovaries of normal rats， while no statistically significant difference was observed across estrous cycle phases （P>0.05）. The immunohistochemistry results showed that the expression of Plet1 protein was mainly localized in ovarian granulosa cells and luteal cells in the rat ovarian tissue. Compared with control group，the expression levels of Plet1 mRNA and protein in ovarian tissue of the rats in PCOS group were significantly decreased （P<0.05）. The RT-qPCR results showed that compared with control group， the expression level of Plet1 mRNA in ovarian granulosa cells in si-Plet1-rat-383 group was decreased （P<0.01）， exhibiting the most pronounced reduction. Compared with control group， the proliferation activity of rat ovarian granulosa cells in si-Plet1-rat-383 group was decreased （P<0.05）. Compared with control group， the expression levels of CDK6 and P53 mRNA in rat ovarian granulosa cells in si-Plet1-rat-383 group were significantly decreased （P<0.05 or P<0.01）. Conclusion Plet1 proteinis predominantly expressed and localized in granulosa cells and luteal cells in normal rat ovarian tissue. Its expression is downregulated in the ovarian tissue of PCOS model rats， and interference with Plet1 gene expression may inhibit the proliferation of rat ovarian granulosa cells.

Key words: Polycystic ovary syndrome, Placenta expressed transcription factor 1, Estrous cycle, Ovarian granulosa cells, Cell proliferation

CLC Number:

R711.7

Lulu FU,Yinggang ZOU,Xiaoyu ZHENG,Xueying ZHANG,Jingshun ZHANG,Min WANG,Qiang ZHANG,Lianwen ZHENG. Expression of placenta expressed transcription factor 1 in ovarian tissue of polycystic ovary syndrome rats and its effect on proliferation of rat ovarian granulosa cells[J].Journal of Jilin University(Medicine Edition), 2025, 51(5): 1177-1184.

Figures/Tables 9

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References 23

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Primer	Sequence（5'-3'）
Plet1	F:AGCGGAGCATACGAGAAGTG
Plet1	R:TGTAGCTGTGCGGTTGATGA
GAPDH	F:CAAGTTCAACGGCACA
GAPDH	R:CAGTAGACTCCACGACAT

Primer	Sequence（5'-3'）
CDK6	F:GCACACATCAAACAACCTGACC
CDK6	R:GGCCCACTTCACCGTACTAA
P53	F:GCTGACCAGCAGTACGAATG
P53	R:GCACACATCAAACAACCTGACC
GAPDH	F:CAAGTTCAACGGCACA
GAPDH	R:CAGTAGACTCCACGACAT

Group	CDK6	P53
Control	1.037±0.080	1.050±0.014
Si-Plet1-rat-383	0.787±0.060^*	0.670±0.057^**

Expression of placenta expressed transcription factor 1 in ovarian tissue of polycystic ovary syndrome rats and its effect on proliferation of rat ovarian granulosa cells

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