The ESCC cells KYSE30， KYSE40 and TE-1 cultured in vitro were used as experimental subjects and divided into control group （0 μmol·L^－1 NVP-AEW541，1 mL RPMI 1640 complete medium + 1 μL DMSO） and experimental groups（2， 4， 6， 8 and 10 μmol·L^－1 NVP-AEW541）.After the cells in control group and experimental group were treated for 48 h， CCK-8 assay was used to detect the cell survival rates in various groups； after the cells in control group and experimental groups （4 and 6 μmol·L^－1 NVP-AEW541）were treated for 0， 6， 12 and 24 h， cell scratch test was used to detect the cell migration rates in various groups； after the cells in control group and experimental group （6 μmol·L^－1 NVP-AEW541） were treated for 48 h， Transwell test was to used detect the number of invasion cells in various groups； after the cells in control group and experimental groups （2， 4 and 6 μmol·L^－1 NVP-AEW541） were treated for 48 h， Western blotting method was used to detect the expression levels of p-IGF-1R and epithelial-mesenchymal transition （EMT）-related proteins N-Cadherin and Snail-1 proteins in the cells in various groups.

Compared with control group， the cell survival rates of KYSE30， KYSE450 and TE-1 cells in experimental groups were decreased with the increase of the concentration of NVP-AEW541 （P<0.05）； the survival rates of KYSE30， KYSE450 and TE-1 cells in experimental group （10 μmol·L^－1） were decreased（P<0.01）； the cell migration rates in experiment group （6 μmol·L^－1） were decreased with the increase of NVP-AEW541 concentration （P<0.01）； the number of invasion cells in experimental group （6 μmol·L^－1） was decreased （P<0.01）； the expression levels of p-IGF-1R，N-Cadherin and Snail-1 proteins in the cells in experimental group were significantly decreased （P<0.01）.

NVP-AEW541 can significantly inhibit the proliferation of ESCC cells，by inhibiting the expressions of N-Cadherin and Snail-1 proteins， it prevents the EMT， and inhibit the migration and invasion of ESCC cells.