Journal of Jilin University(Earth Science Edition) ›› 2020, Vol. 50 ›› Issue (4): 1189-1196.doi: 10.13278/j.cnki.jjuese.20190052

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Bio-Sensing of 17β-Estradiol Using Aptamer Modified Graphene Oxide

Piao Yunxian1,2, Hu Hui1,2, Yao Lan1,2, Zhang Yu1,2, Liang Lina1,2, Liu Zairan1,2   

  1. 1. Key Laboratory of Groundwater Resources and Environment(Jilin University), Ministry of Education, Changchun 130021, China;
    2. College of New Energy and Environment, Jilin University, Changchun 130021, China
  • Received:2019-05-14 Published:2020-07-29
  • Supported by:
    Supported by National Natural Science Foundation of China (51809111)

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Abstract: In order to improve the detection performance of 17β-estradiol in complex environment water, the aptamer-modified graphene oxide complex was synthesized by immobilization of the aptamer on the surface of graphene oxide with direct adsorption,and it was used for high sensitive and specific homogeneous detection of 17β-estradiol contamination in water. The ultraviolet-visible spectroscopy was used to characterize the aptamer-modified graphene oxide complex. It was found that the complex had a wide peak at 250 nm, which proved that the nucleic acid aptamer was successfully immobilized on the surface of graphene oxide. The fluorescence intensity of the aptamer-modified graphene oxide complex before and after reaction with target 17β-estradiol was analyzed by the fluorescence spectrophotometer. It was found that the fluorescence intensities increased from 110 to 450, and the relative fluorescence intensities were proportional to the mass concentrations of 17β-estradiol in the range of 10-100 μg/L with a minimum detection limit of 6.2 μg/L. This indicates that the complex could be successfully used for the detection of 17β-estradiol.

Key words: graphene oxide, aptamer, 17β-estradiol, fluorescence detection, fluorescence resonance energy transfer

CLC Number: 

  • P641
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