关键词: 生物化学工程, 薏苡仁, 灵芝, 液体发酵, 多糖, 灵芝酸, 自由基

Abstract: The effects of Coix lachrymaljobi extract (EEC) on the mycelia growth, bioactive substance production and antioxide activity of Ganoderma lucidum in submerged fermentation were investigated. Addition of EEC at the level of 6 mg·L-1 led the biomass yields to increase from 8.29 ± 0.32 g·L^-1 to 10.19 ± 0.51 g·L^-1. The maximal yields, 0.72 ± 0.03 g·L^-1, of polysaccharides was obtained at EEC concentration of 10 mg·L^-1, and the maximal yields, 0.12±0.01 mg·L^-1, of ganoderic acid was obtained at EEC concentration of 14 mg·L^-1. Comparing with no EEC the increases in biomass, polysaccharides and ganoderic acid yields were 14.6%, 34.21% and 254.2%, respectively. The addition of EEC at the level of 14 mg·L^-1 also enhanced the free radical scavenging activity of Ganoderma lucidum, particularly the activity on superoxide anion radical, and the maximal inhibition rate reached 43.2±0.21%, which is about 61.6% higher than that with no EEC.So EEC could visibly enhance the growth,production and activity of scavenging free radical of Glucidum.

Key words: biochemistry engineering, Coix lachrymajobi, Ganoderma lucidum, submerged fermentation, polysaccharides, ganoderic acid, free radical