基于斯托克位移的定量荧光强度测试仪

吉林大学学报(工学版) ›› 2002, Vol. ›› Issue (4): 66-69.

基于斯托克位移的定量荧光强度测试仪

张赤军¹, 吴宝宁², 宋一兵²

1. 长春理工大学, 科技处, 吉林, 长春, 130022;
2. 西安应用光学研究所, 国防科工委光学计量一级站, 陕西, 西安, 710065

收稿日期:2002-03-17

Quantitative Fluorometry Based on Stoke's Shift

ZHANG Chi-jun¹, WU Bao-ning², SONG Yi-bing²

1. Office of Science and Technology, Changchun University of Science and Technology, Changchun 130022, China;
2. Xi'an Institute of Applied Optics, The First Scale Optical Metrology Station of Commission of Science, Technology and Industry for National Defence, Shanxi Xi'an 710065, China

Received:2002-03-17

摘要/Abstract

摘要：

详细论述了荧光发生斯托克位移(Stoke's shift)的机理,运用荧光的斯托克位移现象确定激发光源的波长范围,为干涉滤光片的镀膜工艺提供了依据.并介绍了新近研制成功的医用定量荧光强度测试仪的测量原理、数学模型以及光学上的关键技术.最后指出了该仪器的应用前景.

关键词: 荧光, 荧光强度, 斯托克位移, 血循环诊断

Abstract:

Principle of fluorescence of Stoke's shift is discussed in details.Based on the principle,range of exciting wavelength is determined which may improve coating technology.Accordingly,a recently developed fluorometry and it's measured principle,mathematics model,key technology of optics are described.Moreover,the future application of fluorometry is pointed.

Key words: fluorescence, fluorescent intensity, Stoke’s shift, blood circle diagnoses

中图分类号:

TN98

张赤军, 吴宝宁, 宋一兵. 基于斯托克位移的定量荧光强度测试仪[J]. 吉林大学学报(工学版), 2002, (4): 66-69.

ZHANG Chi-jun, WU Bao-ning, SONG Yi-bing. Quantitative Fluorometry Based on Stoke's Shift[J]. 吉林大学学报(工学版), 2002, (4): 66-69.

参考文献

[1] Lange K,Krewer S E.The demofluorometer［J］.J.lab Clin Med.,1943,28:1746～1751.
[2] Silverman D G,Cedrone F A,Harford we,et al.Monitoring tissue elimination of fluorescence with the perfusion fluorometer:A new method to assess capillary blood flow［J］.Surgery,1981,90:409～417.
[3] Jeffey Copeland,Roger M D,Harman D.Quantitive fluorometry as a monitor for microvascular surgery［J］.The Journal of Hard Surgery,1987,12A(2):250～255.
[4] 樊美公.光化学基本原理与光子学材料科学［M］.北京:北京科学出版社,2001.

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