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Active Components and Antioxidant Effectof Perilla stem Extract by HPLC
ZHAGN Lei, LI Qiushuang, HAO Jingwei, WAN Chunlei, JING Yunrong, CHAI Junhong, JI Qiuyan
Journal of Jilin University Science Edition. 2020, 58 (4):
1020-1025.
The effective components of Perilla stem were extracted by ethyl acetate, chloroformane, nbutanol and water respectively, the organic acids, triterpenoids and flavonoids were obtained, and its antioxidant effect was studied. The results show that the gallic acid is 0.6 g/kg in n-butyl alcohol of Perilla stem, and the oleanolic acid is 0.9 g/kg in chloroform of Perilla stem. There are lots of brass compounds in Perilla stem, and the hyperin are 1.2 g/kg in ethyl acetate and 1.1 g/kg in chloroform. All of the Perilla stem extracts have certain ability to remove ·OH, the super oxygen free radical (·O-2) and DPPH·. The removal ability of acetic ether and chloroform in 1 mg/mL are best, which reach 79% and 73%, 62% and 59%, 83% and 80% respectively. Acetic ether and chloroform have strong ability to remove ·OH radicals and DPPH·. Therefore Perilla stem extract has obvious antioxidant effect.
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