吉林大学学报(医学版)

• 基础研究 • 上一篇    下一篇

硫化氢对高肺血流性肺动脉高压大鼠平滑肌细胞线粒体
损伤的保护作用及其机制

刘军杰,黄国雄,黎 静,张 星,谢 露   

  1. 广西医科大学基础医学院生理学教研室,广西 南宁 530021
  • 收稿日期:2013-09-06 出版日期:2014-05-28 发布日期:2014-06-05
  • 通讯作者: 谢 露(Tel:0771-5355280,E-mail:xielu@163.com) E-mail:xielu@163.com
  • 作者简介:刘军杰(1978-),女,山东省淄博市人,医学博士,在站医学博士后,主要 从事心血管的细胞形态学研究。 
  • 基金资助:

     广西壮族自治区科技厅自然科学基金资助课题(2013GXNSFAA019215)

Protective effect of hydrogen sulfide on mitochondrial damage
of smooth muscle cells of rats with high pulmonary blood flow 
pulmonary hypertension and its mechanism

LIU Jun-jie,HUANG Guo-xiong,LI Jing,ZHANG Xing,XIE Lu   

  1. Department of Physiology,School of Basic Medical Sciences,Guangxi Medical University,Nanning 530021,China
  • Received:2013-09-06 Online:2014-05-28 Published:2014-06-05

摘要:

目的:探讨硫化氢(H2S)对高肺血流性肺动脉高压大鼠肺动脉平滑肌细胞线粒体功能的影响,阐明其在高肺血流性肺动脉高压形成中的作用及其机制。方法:将27只大鼠随机分为假手术组、手术组和手术+硫氢化钠(NaHS)组,每组9只。行左肺切除术建立大鼠肺动脉高压模型。饲养35 d后,分别测定3组大鼠肺动脉平均压(mPAP),检测右心室/ 体质量(RV/BW) 和右心室/(左心室+ 室间隔)[RV/(LV + S)] 比值。检测各组大鼠血浆H2S水平和肺动脉组织中硫化氢还原酶(CSE)活性;测定线粒体总ATP酶、超氧化物歧化酶(SOD)、谷甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)水平;透视电镜观察肺动脉平滑肌线粒
体超微结构。结果:与假手术组比较,手术组大鼠血浆H2S水平和肺动脉组织中CSE活性降低(P< 0.01);肺动脉组织线粒体膜肿胀,线粒体活力下降(P<0.01);线粒体中ATP 酶、SOD、GSH-Px 活性明显降低,MDA水平明显升高(P<0.01) 。与手术组比较,手术+NaHS组大鼠血浆H2S 水平和肺动脉组织中CSE 活性均升高( P< 0.01); 线粒体膜肿胀度减轻,活力有所恢复; 肺动脉组织中 ATP 酶、GSH-Px和SOD的活性明显升高( P< 0.01),MDA水平明显减少( P<0.01)。结论:H2S可增强线粒体ATP 酶、GSH-Px和SOD的活性,降低线粒体脂质过氧化水平,起到对大鼠肺动脉平滑肌的保护作用。

关键词: 硫化氢, 高肺血流, 肺动脉高压, 平滑肌, 线粒体

Abstract:

Objective To study the influence of hydrogen sulfide (H2S) on the  mitochondrial function of pulmonary artery smooth muscle cells of the  rats with high pulm
onary blood flow pulmonary hypertension,and to clarify the function and its mechanism in the occurrence of  high pulmonary blood flow pulmonary hypertension.Methods A total of 27 rats were randomly divided sham operation group,operation group,and operation+sodium hydrosulfide (NaHS) group (n=9).The pulmonary hypertension rat model was built by left lung resection.After fed for 35 d,the mean pulmonary artery pressure (mPAP),ratio of right ventricle / body weight (RV / BW),and ratio of right ventricle /(left ventricle+septum) [RV /(LV + S)] of the rats in three groups were measured.The plasma H2S levels and the CSE activities in pulmonary artery tissue of the rats were detected;the activities of total mitochondrial ATP enzyme,superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) levels were determined;the ultrastructure of pulmonary artery smooth muscle mitochondria was observed through transmission electron microscope.Results  Compared with sham operation group,the plasma H2S level and the CSE activity in p
ulmonary artery tissue of the rats in operation group  were decreased(P<0.01);the mitochondrial membrane of pulmonary tissue was swelling,and the mitochondrial activity was decreased(P<0.01);the mitochondrial ATP enzyme,SOD and GSH-Px activities were significantly decreased,and the MDA level was significantly increased (P<0.01).Compared with operation group,the H2S level in  plasma and the activity of CSE in pulmonary tissue of the rats in operation + NaHS group were increased(P<0.01);the mitochondrial membrane swelling was reduced,and the vitality was restored;the ATP enzyme,GSH-Px,and SOD level in pulmonary tissue were significantly increased (P<0.01),and the MDA level was significantly reduced(P<0.01).Conclusion H2S can enhance the activities of mitochondrial ATP enzyme,GSH-Px,and SOD,and decrease the mitochondrial lipid peroxidation level,thus it plays a protective effect on  rat pulmonary artery smooth muscle.

Key words: hydrogen sulfide, high pulmonary blood flow, pulmonary hypertension, smooth muscle, mitochondria

中图分类号: 

  •