吉林大学学报(医学版)

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壳层隔绝纳米粒子增强拉曼光谱检测乳腺浸润性导管
癌组织的生物学特点及其临床意义

张海鹏1，吴 迪2，张 湜*,付 彤2，路 璐2，范志民2，郑 超2，韩 冰2   

  1. 1.   吉林大学第一医院产科，吉林 长春 130021；2.吉林大学第一医院乳腺外科，吉林
     长春 130021
  • 收稿日期:2014-02-10 出版日期:2014-09-28 发布日期:2014-11-24
  • 通讯作者: 韩 冰（Tel：0431-81875672，E-mail：yintian77@126.com）； 付 彤(Tel：0431-88782963，E-mail：xxli@jlu.edu.cn) E-mail:yintian77@126.com；xxli@jlu.edu.cn
  • 作者简介:张海鹏（1980-），女，吉林省长春市人，主治医师，在读医学博士，主要从事 生物拉曼光谱的研究。
  • 基金资助:

    国家自然科学基金青年基金资助课题(81202078)；吉林省科技厅科技发展计划青年
    基金资助课题(20130522030JH)；吉林省科技厅科研基金资助课题(201015155)

Biological characteristics of breast invasive ductal 
carcinoma detected by shell-isolated nanoparticle-
enhanced Raman spectroscopy and their clinical significances

ZHANG Hai-peng1,WU Di2,ZHANG Shi*,FU Tong2,LU Lu2,FAN Zhi-min2,ZHENG Chao2,HAN Bing2   

  1. 1.Department of Obstetrics Surgery,First Hospital,Jilin University,Changchun 130021,China；
    2.Department of Breast Surgery,First Hospital,Jilin University,Changchun 130021,China
  • Received:2014-02-10 Online:2014-09-28 Published:2014-11-24

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摘要：

目的：采用壳层隔绝纳米粒子增强拉曼光谱(SHINERS)技术检测乳腺浸润性导管癌(IDC)组织和正常乳腺组织，探讨乳腺IDC的光谱学特点、生物学特征和鉴别方法。方法：收集行乳腺外科手术患者的乳腺组织冰冻切片，共24例，均为女性，年龄27~59岁；其中乳腺IDC组织15例，正常乳腺组织9例。冰冻切片解冻后先行普通拉曼光谱检测，加壳层隔绝纳米粒子(SHINs)后再次检测。共收集了263个拉曼光谱和249个SHINERS光谱，所有光谱均进行基线修正拟合，再将所有的光谱用Adjacent-Averaging算法进行15点平滑。结果：正常乳腺组织特征峰出现在1 090、1 157、1 262、1 300、1 442、1 658、1 745和1 874 cm-1；在加入SHINs后，少数特征峰的峰位出现2~3 cm-1位移，其中1 090和1 157 cm-1相对强度明显增加，出现1 496 cm-1特征峰。乳腺IDC组织普通拉曼光谱检测可见多个核酸特征峰(包括878、1 086和1 157 cm-1)；加入SHINs后，明显看到1 004、1 157、1 526和1 658 cm-1相对强度增加，脂类的特征峰1 745和1 442 cm-1为C=O和CH2伸缩振动，IDC组织相对于正常组织表现出2~3 cm-1的蓝移；类胡萝卜素的特征峰出现在1　527 cm-1；核酸的特征峰1 090 cm-1蓝移至1 086 cm-1
。结论：拉曼光谱能够发现乳腺IDC组织DNA、蛋白质及类胡萝卜素与正常乳腺组织的差异。SHINERS对不同类型的乳腺组织最大增强的特征峰不同，可以用来区分乳腺IDC组织和正常乳腺组织。

关键词: 乳腺肿瘤, 拉曼光谱, 表面增强拉曼光谱, 浸润性导管癌

Abstract:

Objective To identify normal breast tissue and breast invasive ductal carcinoma (IDC) tissue by shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS),and to explore the biological characteristics of IDC and the identification method by discussing its spectroscopy characteristics.
Methods The frozen section of breast tissue from 24 patients (female,aged 27-59 years) underwent routine surgical resection were obtained.9 cases of normal breast tissue and 15 cases of IDC breast tissue were detected by Raman spectroscopy and then SHINERS technique was utilized.A total of 263 Raman spectra and 249 SHINERS spectra were obtained.All the spectra were dealt with baseline corrected by fitting and subtracting a third-order polynomial and then smoothed with a 15-point Adjacent-Averaging.Results The characteristic peak of normal breast tissue appeared at 1 090,1 157,1 262,1 300,1 442,1 658,1 745,and 1 874 cm-1.After adding shell-isolated nanoparticles (SHINs),some peaks shifted at 2-3 cm-1,and the relative strengthes of 1 090 and 1 157 cm-1 were significantly increased,and the  characteristic peak at 1 496 cm-1 appeared.The Raman spectroscopy showed there were more nucleic acid ch
aracteristic peaks (including 878,1 086,1 157 cm-1) in the breast IDC tiss
ue;
after
adding SHINs,the relative strengthes of 1 004,1 157,1 526,and 1 658 cm-1 were  increased,the characteristic peak of lipid at 1 745 cm-1 and 1 442 cm-1
 stemmed from the C=O and CH2 stretching vibration.Compared with the normal breast tissue,the breat IDC tissue showed a blue shift of 2-3 cm-1.The characteristic peaks of nucleic acid had the blue shift from 1 086 cm-1 to 1 090 cm-1,and the characteristic peak of β-carotene emerged at 1 527 cm-1.
Conclusion Raman spectra can discover the differences of the characteristic peaks of DNA,β-carotene,and protein between breast IDC and normal breast tissues;SHINERS can distinguish breast IDC tissue from normal breast tissue successfully.

Key words: breast neoplasms, Raman spectroscopy, surface enhanced Raman spectroscopy, invasive ductal carcinoma

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