富血小板血浆对创伤性颅脑损伤大鼠神经功能的保护作用

doi:10.13481/j.1671-587x.20160514

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (05): 910-914.doi: 10.13481/j.1671-587x.20160514

富血小板血浆对创伤性颅脑损伤大鼠神经功能的保护作用

王亚东¹, 李东朋¹, 郭德伟¹, 宋及时², 李红伟¹, 钱伟强¹, 杨波¹

1. 郑州大学第一附属医院神经外科, 河南郑州 450052;
2. 郑州大学生命科学学院干细胞研究室, 河南郑州 450052

收稿日期:2015-11-05 出版日期:2016-09-28 发布日期:2016-09-29
通讯作者: 杨波,主任医师,教授,博士研究生导师(Tel:0371-67967096,E-mail:yangbo96@126.com) E-mail:yangbo96@126.com
作者简介:王亚东(1991-),男,河南省南阳市人,在读医学硕士,主要从事神经外科基础与临床方面的研究。
基金资助:
河南省教育厅高校科技创新团队资助课题（15IRTSTHN022）

Protective effect of platelet-rich plasma on never function in rats with traumatic brain injury

WANG Yadong¹, LI Dongpeng¹, GUO Dewei¹, SONG Jishi², LI Hongwei¹, QIAN Weiqiang¹, YANG Bo¹

1. Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China;
2. Stem Cell Laboratory, School of Life Science, Zhengzhou University, Zhengzhou 450001, China

Received:2015-11-05 Online:2016-09-28 Published:2016-09-29

摘要/Abstract

摘要：

目的：探讨富血小板血浆（PRP）对创伤性颅脑损伤（TBI）大鼠神经功能的保护作用，阐明其作用机制。方法：60只SD大鼠随机分为假手术组（仅开放骨窗）、TBI模型组（TBI组）和TBI模型并PRP处理组（PRP组），每组20只。PRP组大鼠于术后当天、第2和6天血管内注射PRP，TBI组和假手术组大鼠注射生理盐水。3组大鼠分别于术后第1、3和7天时行改良大鼠神经功能缺损（mNSS）评分；每组各取10只大鼠处死并取脑，制作切片行组织学观察，剩余大鼠行Morris水迷宫实验。结果：与假手术组比较，TBI组和PRP组大鼠mNSS评分明显升高（P<0.05）；与TBI组比较，PRP组大鼠mNSS评分明显降低（P<0.05）；与TBI组比较，PRP组大鼠脑组织损伤体积明显减小（P<0.05）；Nissl染色，PRP组大鼠脑损伤区细胞排列相对整齐，新生毛细血管多于TBI组；免疫组织化学染色，与假手术组比较，TBI组大鼠脑损伤区域有较多胶质纤维酸性蛋白阳性（GFAP⁺）细胞，但神经元核抗原阳性（neuN⁺）细胞少于PRP组（P<0.05）。Morris水迷宫实验，PRP组大鼠在各象限逃避潜伏期均低于TBI组（P<0.05），穿越平台次数及第三象限游泳时间高于TBI组（P<0.05）。结论：PRP对TBI大鼠可发挥神经保护作用。

关键词: 创伤性颅脑损伤, 富血小板血浆, 神经功能

Abstract:

Objective: To study the protective effect of platelet-rich plasma on never function in the rats with traumatic brain injury(TBI),and to clarify its mechanisms. Methods: Sixty SD rats were randomly divided into sham group(opened skull bone window only), TBI group and platelet-rich plasma treatment group(PRP group)(n=20). The rats in PRP group were injected with platelet-rich plasma through vessel on the 1st day, the 2nd day and the 6th day after operation while the rats in sham group and TBI group were treated with saline at the same time. The neurological function defects were assessed with modified neurological severity score(mNSS) on the 1st, 3rd and 7th after operation. Then 10 rats were taken from each group and executed, and the brain tissues were taken. The brain sections were prepared for the histological observation and the others of each group were tested with Morris water maze. Results: Compared with sham group,the mNSS scores of the rats in TBI and PRP group were increased (P<0.05); the mNSS score of the rats in PRP group was decreased compared with TBI group(P<0.05). The injured volume of rat brain tissue was reduced significantly in PRP treated group compared with TBI group (P<0.05). The Nissl staining results showed that the injury area in PRP group had a more neat rows and a larger number of new blood vessels compared with TBI group. The immunohistochemical staining results showed the injured area had a higher level expression of GFAP⁺ cells in TBI group compared with PRP group, but the amount of neuN⁺ cells was smaller than that in PRP group(P<0.05). The Morris water maze test results showed that there were a shorter escape latency time, more times acrossing platform and a larger swimming time during platform quadrant in PRP group compared with TBI group(P<0.05). Conclusion: Platelet-rich plasma has a significant role in protecting the neurological function of TBI rats.

Key words: traumatic brain injury, platelet-rich plasma, neurological function

中图分类号:

R651.15

王亚东, 李东朋, 郭德伟, 宋及时, 李红伟, 钱伟强, 杨波. 富血小板血浆对创伤性颅脑损伤大鼠神经功能的保护作用[J]. 吉林大学学报(医学版), 2016, 42(05): 910-914.

WANG Yadong, LI Dongpeng, GUO Dewei, SONG Jishi, LI Hongwei, QIAN Weiqiang, YANG Bo. Protective effect of platelet-rich plasma on never function in rats with traumatic brain injury[J]. Journal of Jilin University Medicine Edition, 2016, 42(05): 910-914.

参考文献

[1] Angels Font M, Arboix A, Krupinski J. Angiogenesis, neurogenesis and neuroplasticity in ischemic stroke[J]. Curr Cardiol Rev, 2010,6(3):238-244.

[2] Boswell SG, Cole BJ, Sundman EA, et al. Platelet-rich plasma:a milieu of bioactive factors[J]. Arthroscopy, 2012,28(3):429-439.

[3] Zhang Y, Ying G, Ren C, et al. Administration of human platelet-rich plasma reduces infarction volume and improves motor function in adult rats with focal ischemic stroke[J]. Brain Res, 2015,1594:267-273.

[4] 张荣军, 游潮, 蔡博文, 等. Feeney法建立大鼠闭合性脑损伤模型及评估[J]. 中国修复重建外科杂志, 2005,19(12):1015-1018.

[5] Landesberg R, Roy M, Glickman RS. Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation[J]. J Oral Maxillofac Surg, 2000,58(3):297-300;discussion 300-1.

[6] 吕敏, 裴国献, 刘勇, 等. 富血小板血浆的制备现状及研究进展[J]. 现代生物医学进展, 2013,13(13):2574-2577.

[7] Lu D, Goussev A, Chen J, et al. Atorvastatin reduces neurological deficit and increases synaptogenesis, angiogenesis, and neuronal survival in rats subjected to traumatic brain injury[J]. J Neurotrauma, 2004,21(1):21-32.

[8] Narayan RK, Maas AI, Servadei F, et al. Progression of traumatic intracerebral hemorrhage:a prospective observational study[J]. J Neurotrauma, 2008,25(6):629-639.

[9] Takeuchi M, Kamei N, Shinomiya R, et al. Human platelet-rich plasma promotes axon growth in brain-spinal cord coculture[J]. Neuroreport, 2012,23(12):712-716.

[10] Shen J, Ishii Y, Xu G, et al. PDGFR-β as a positive regulator of tissue repair in a mouse model of focal cerebral ischemia[J]. J Cereb Blood Flow Metab, 2012,32(2):353-367.

[11] Haas SL, Fitzner B, Jaster R, et al. Transforming growth factor-beta induces nerve growth factor expression in pancreatic stellate cells by activation of the ALK-5 pathway[J]. Growth Factors, 2009,27(5):289-299.

[12] Pandya NM, Dhalla NS, Santani DD. Angiogenesis-a new target for future therapy[J]. Vascul Pharmacol, 2006,44(5):265-274.

[13] 张源, 张文进, 田毅, 等. 大鼠创伤性颅脑损伤程度与血清MCP-1、VEGF及损伤区CD34⁺细胞表达水平的关系[J]. 中华神经医学杂志, 2014,13(3):224-228.

[14] Lee SC, Lee KY, Kim YJ, et al. Serum VEGF levels in acute ischaemic strokes are correlated with long-term prognosis[J]. Eur J Neu, 2010,17(1):45-51.

[15] Yates D. Traumatic brain injury:Serum levels of GFAP and S100B predict outcomes in TBI[J]. Nat Rev Neurol, 2011,7(2):63.

[16] Preusser M, Laggner U, Haberler C, et al. Comparative analysis of NeuN immunoreactivity in primary brain tumours:conclusions for rational use in diagnostic histopathology[J]. Histopathology, 2006,48(4):438-444.

[17] Zhang RSS, Xue YSS, Lu SSS, et al. Bcl-2 enhances neurogenesis and inhibits apoptosis of newborn neurons in adult rat brain following a transient middle cerebral artery occlusion[J]. Neurobiol Dis, 2006,24(2):345-356.

富血小板血浆对创伤性颅脑损伤大鼠神经功能的保护作用

Protective effect of platelet-rich plasma on never function in rats with traumatic brain injury

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