关键词: 牙周膜细胞, 胶原, 聚乳酸/聚羟基乙酸共聚体, 富血小板血浆, 牙周组织再生 ,  

Abstract:

Abstract:Objective
To make complex biomembrane with collagen (Co),polylactic acid / polyglycolic acid copolymer (PLGA),periodontal ligament cells (PDLCs) and autologous platelet gel (APG) and to evaluate the repair effects on  periodontal tissue defect of the rats,and to provide theoretical basis for their clinical application.Methods Synthetic PLGA,human PDLCs and PLGA were co-cultured in vitro for 3 d,10 mL whole blood of the rats was extracted to make platelet-rich plasma (PRP),and the growth factor levels in activated PRP andhole blood were detected.10%CaCl2 including 1 000 U•mL^-1 bovine thrombin was added to make APG.60 Wistar adult male rats were randomly divided into 5 groups,and the mandible alveolar bone defect models were established (5 mm×2 mm，root cementums were struck off),the 5 groups were separately named control group,PLGA+Co group,PLGA+APG+Co group,PLGA+PDLCs+Co group,and PLGA+PDLCs+APG+Co group   for the stuff placed into mandible alveolar bones’ defects.Then 4 rats in each group were exeacted at  the 2nd week,the 4th week and the 6th week after operation,and HE staining was used to observe the quantity of new born bones.Image-Pro Plus was used to obtain the areas of new bones,and SPSS 13.0 was used for  statistics analysis.Results The average pore size of PLGA was (131.80±43.97)  μm and the porosity was 60%.PDLCs stretched well on PLGA.The number of platelets in PRP were 4 times higher than those in whole blood and the growth factor levels in supernatant were higher than those in blood plasma.The quantity of new bones at the 6th week after operation in PLGA+PDLCs+APG+Co group was  more than those in PLGA+Co group,PLGA+APG+Co group and PLGA+PDLCs+Co group(P<0.05);the quantities of new bones in PLGA+APG+Co group and PLGA+PDLCs+Co group were more than that in  PLGA+Co group;the quantities of new bones in PLGA+Co group,PLGA+APG+Co group,PLGA+PDLCs+Co group and PLGA+PDLCs+APG+Co group were more than that in control group(P<0.05),
but there was no significant difference between PLGA+APG+Co group and  PLGA+PDLCs+Co group(P>0.05).Conclusion The pure size PLGA has excellent biocompatibility,suitable high porosity and good mechanical properties.PLGA+PDLCs+APG+Co complex biomembrane plays significant role in promoting periodontal tissue growth.
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Key words: periodontal ligament cells, collagen, polylactic-co-glycolic acid,
platelet-rich plasma, periodontal tissue regeneration