关键词: 狂犬病病毒街毒株, 神经元, 神经功能异常, C57/BL小鼠

Abstract:

Abstract:Objective To discuss the morphological changes of neurons infected with street strain of rabies virus(RV), and to explore the mechanism of the neuronal dysfunction caused by RV.Methods In vivo,the C57/BL mice were randomly divided into experimental group(n=10) and control group(n=10).The mice in experimental group were inoculated intracranially at 30 μL 　RV　 suspension (10TCID50),and the mice in control group were inoculated
 with identical volume of cell maintenance medium.Anti-ＲＶ antibody was used to detect the RV antigen distribution in brain tissue of the mice.In vitro,the primary hippocampal neurons were cultured for 1 week and the cells were infected by RV.The proliferation of RV was  detected by immunofluorescence method after infection for 72,96, and 120 h.Results The RV  antigen was found in neurons and neuronal dendrites  of hippocampus CA1  4 d after infection;however,the RV antigen was detected only in neuronal dendrites of hippocampus CA1  7 d after infection.The number of infected neurons was the most 120 h after infection,and the number of infected dendrites was decreased. Conclusion RV can  infect and injure the neuronal   dendrites of  hippocampus CA1 region and lead to neuronal dysfunction of the mice.

Key words: street strain of rabies virus, neuron；neuronal dysfunction；C57/BL mice