灵芝酸A对人炎性乳腺癌细胞体外增殖和凋亡的影响

doi:10.13481/j.1671-587x.20180122

吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (01): 116-120.doi: 10.13481/j.1671-587x.20180122

灵芝酸A对人炎性乳腺癌细胞体外增殖和凋亡的影响

林晓萌¹, 丁琪琼², 陈鹊汀¹, 张磊³, 郝鑫^1,3, 李中¹

1. 河北大学附属医院乳腺外科, 河北保定 071000;
2. 北京协和医院临床与药理中心, 北京 100032;
3. 河北大学附属医院肿瘤内科河北省肿瘤放化疗机制与规程研究重点实验室, 河北保定 071000

收稿日期:2017-05-19 出版日期:2018-01-28 发布日期:2018-01-24
通讯作者: 李中,主任医师,硕士研究生导师(Tel:0312-5981262,E-mail:xiaomenglxm@163.com) E-mail:xiaomenglxm@163.com
作者简介:林晓萌(1981-),男,河北省保定市人,主治医师,医学硕士,主要从事乳腺癌细胞分子生物学方面的研究。
基金资助:
河北省卫计委医学科学研究项目资助课题（20160045）；河北省保定市科技局科技支撑计划项目资助课题（17ZF124）

Effects of ganoderma lucidum A on proliferation and apoptosis of human inflammatory breast cancer cells in vitro

LIN Xiaomeng¹, DING Qiqiong², CHEN Qiaoting¹, ZHANG Lei³, HAO Xin^1,3, LI Zhong¹

1. Department of Breast Surgery, Affiliated Hospital, Hebei University, Baoding 071000, China;
2. Clinical and Pharmacological Center, Peking Union Medical College Hospital, Beijing 100032, China;
3. Department of Oncology, Affiliated Hospital, Hebei University, Key Laboratory of Mechanism and Regulation of Tumor Radiotherapy and Chemotherapy in Hebei Province, Baoding 071000, China

Received:2017-05-19 Online:2018-01-28 Published:2018-01-24

摘要/Abstract

摘要： 目的：探讨灵芝酸A在体外对人炎性乳腺癌SUM149细胞增殖和凋亡的影响，阐明其可能机制。方法：人炎性乳腺癌SUM149细胞分为对照组和不同浓度（0.1、0.5、1.0、5.0和10.0 μmol·L^-1）灵芝酸A组，每组设4个复孔，分别于培养24、48和72h取样本。MTT法检测各组SUM149细胞增殖抑制率，流式细胞术检测各组SUM149细胞凋亡率和细胞周期，免疫细胞化学染色法检测各组SUM149细胞Ki67和Livin蛋白表达水平。结果：MTT法检测，与0.1μmol·L^-1灵芝酸A组比较，其他浓度灵芝酸A组SUM149细胞增殖抑制率明显升高（P<0.05或P<0.01），并呈浓度和时间依赖性。流式细胞术检测，与对照组比较，不同浓度灵芝酸A组SUM149细胞凋亡率明显升高（P<0.05或P<0.01），并呈浓度和时间依赖性；同时细胞周期发生明显变化，与对照组比较，随着灵芝酸A浓度的增加和作用时间的延长，不同浓度灵芝酸A组G₁期细胞百分率明显增加（P<0.05），S期细胞百分率减少（P<0.05或P<0.01）。与对照组比较，不同浓度灵芝酸A组细胞中Ki67和Livin蛋白表达水平降低（P<0.05或P<0.01）。结论：灵芝酸A通过诱导细胞凋亡抑制人炎性乳腺癌SUM149细胞增殖。

关键词: 人炎性乳腺癌细胞, 灵芝酸A, 细胞增殖, 细胞凋亡

Abstract: Objective: To study the effects of ganoderma lucidum A on the proliferation and apoptosis of human inflammatory breast cancer SUM149 cells in vitro,and to clarify their mechanisms. Methods: The human inflammatory breast cancer SUM149 cells were divided into control group and different concentrations (0.1,0.5,1.0,5.0 and 10.0 μmol·L^-1) of ganoderma lucidum A groups; four holes were set up,and the samples were taken at 24,48 and 72 h after culture.MTT assay was used to detect the inhibitory rate of proliferation of SUM149 cells.Flow cytometry was used to detect the apoptotic rate and cell cycle of SUM149 cells.The expression levels of Ki67 and Livin proteins in SUM149 cells were detected by immunocytochemical staining. Results: Compared with 0.1 μmol·L^-1 ganoderma lucidum A group,the inhibitory rates of proliferation of SUM149 cells in other concentrations of ganoderma lucidum A groups were significantly increased detectde by MTT method (P<0.05 or P<0.01) in a concentration-and time-dependent manner.The flow cytometry results showed that the apoptotic rates of SUM149 cells in different conventrations of ganoderma lucidum A groups were significantly higher than that in control group (P<0.05 or P<0.01) in a concentration-and time-dependent manner;at the same time,the cell cycle changed significantly.With the increase of ganoderma lucidum A of the concentration of ganoderma lucidum A,the pencentages of cells at G₁ phase in different concentrations of ganoderma lucidum A groups were increased(P<0.05),and the percentages of cells at S phase were decreased compared with control group(P<0.05 or P<0.01).The expression levels of Ki67 and Livin proteins in different concentrations of ganoderma lucidum A groups were decreased compared with control group (P<0.05). Conclusion: Ganoderma lucidum A can inhibit the proliferation of human inflammatory breast cancer SUM149 cells through induction of apoptosis.

Key words: human inflammatory breast cancer cells, ganoderma lucidum A, cell proliferation, apoptosis

中图分类号:

R737.9

林晓萌, 丁琪琼, 陈鹊汀, 张磊, 郝鑫, 李中. 灵芝酸A对人炎性乳腺癌细胞体外增殖和凋亡的影响[J]. 吉林大学学报(医学版), 2018, 44(01): 116-120.

LIN Xiaomeng, DING Qiqiong, CHEN Qiaoting, ZHANG Lei, HAO Xin, LI Zhong. Effects of ganoderma lucidum A on proliferation and apoptosis of human inflammatory breast cancer cells in vitro[J]. Journal of Jilin University Medicine Edition, 2018, 44(01): 116-120.

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灵芝酸A对人炎性乳腺癌细胞体外增殖和凋亡的影响

Effects of ganoderma lucidum A on proliferation and apoptosis of human inflammatory breast cancer cells in vitro

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