吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (02): 300-306.doi: 10.13481/j.1671-587x.20190215

• 基础研究 • 上一篇    

4种品系五味子木脂素类成分的含量及功效比较

梁爽1, 陶雪1, 潘炎1, 苑荣爽1, 李贺1, 孙靖辉1, 鞠文博2, 陈建光1, 王春梅1   

  1. 1. 北华大学药学院药理学教研室, 吉林 吉林 132013;
    2. 北华大学基础医学院组织胚胎学教研室, 吉林 吉林 132013
  • 收稿日期:2018-04-18 发布日期:2019-03-29
  • 通讯作者: 陈建光,教授,硕士研究生导师(Tel:0432-64608281,E-mail:chenjg@beihua.edu.cn);王春梅,教授,硕士研究生导师(Tel:0432-64608281,E-mail:wangcm74@126.com) E-mail:chenjg@beihua.edu.cn;wangcm74@126.com
  • 作者简介:梁爽(1993-),女,吉林省长春市人,在读医学硕士,主要从事中药五味子的基础与开发方面的研究。
  • 基金资助:
    吉林省科技厅产业技术联盟项目资助课题(20150309004YY,20170309006YY);吉林省科技厅医药产业推进计划项目资助课题(201603103YY)

Comparison of contents and effects of lignans from 4 strains of Schisandra chinensis

LIANG Shuang1, TAO Xue1, PAN Yan1, YUAN Rongshuang1, LI He1, SUN Jinghui1, JU Wenbo2, CHEN Jianguang1, WANG Chunmei1   

  1. 1. Department of Pharmacology, College of Pharmacy, Beihua University, Jilin 132013, China;
    2. Department of Histology and Embryology, College of BasicMedical Sciences, Beihua University, Jilin 132013, China
  • Received:2018-04-18 Published:2019-03-29

摘要: 目的:比较4种不同品系五味子木脂素类成分含量及相关功效的差异,为精准选用五味子品种提供实验依据。方法:采用高效液相色谱法(HPLC)检测4种品系五味子中7种木脂素单体的含量;通过体外实验测定还原能力、清除DPPH自由基能力及抑制亚油酸氧化能力,比较4种五味子的抗氧化作用强度。60只雄性ICR小鼠随机分为正常对照组、酒精性肝损伤模型组(模型组)、五味子嫣红(YH)组、SH组、156-5-2组和50-1-5组,每组10只。末次给药1 h后,正常对照组小鼠给予同体积蒸馏水,其余组小鼠均灌胃给予50%酒精(12 mL·kg-1),禁食不禁水。12 h后处死,取小鼠肝脏和血清,测定小鼠血清中丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)水平,测定小鼠肝组织中超氧化物歧化酶(SOD)活性和甘油三酯(TG)及丙二醛(MDA)水平。结果:五味子YH的木脂素7种单体总含量最高,其次为五味子SH、156-5-2和50-1-5;除156-5-2外,其余3种五味子均具有较强的抗氧化作用,强度顺序为YH>SH>50-1-5。与模型组比较,五味子YH组小鼠血清ALT和AST水平及肝组织中MDA和TG水平均明显降低(P<0.05或P<0.01);SH组小鼠肝组织中TG水平降低(P<0.05),但ALT和AST水平变化不明显(P>0.05);156-5-2组小鼠血清ALT水平降低(P<0.05),但肝组织中MDA和TG水平无明显变化(P>0.05)。结论:在4种品系五味子中,YH的木脂素含量最高,体外抗氧化效果及对急性酒精性肝损伤的保护作用最好。

关键词: 五味子, 木脂素, 抗氧化, 酒精性肝损伤

Abstract: Objective: To campare the differences of contents and relative effects of lignans from 4 strains of Schisandra chinensis, and to provide the experimental basis for the precise selection and application.Methods: The contents of 7 kinds of lignans from 4 strains of Schisandra chinensis were determined by high performance liquid chromatography (HPLC). The antioxidant effects of 4 strains of Schisandra chinensis were compared by measuring the abilities of reduction, scavenging of DPPH free radical and inhibiting linoleic acid oxidation in vitro. Sixty male ICR mice were randomly divided into normal control group, alcoholic liver injury model group (model group),Schisandra chinensis YH group, (YH group), Schisandra chinensis SH group (SH group),Schisandra chinensis 156-5-2 group(156-5-2 group) and Schisandra chinensis 50-1-5 group (50-1-5 group), 10 mice in each group. One hour after the last administration, the mice in normal control group were given the equal volume of distilled water, and those in the other groups were intragastrically given 50% alcohol (12 mL·kg-1). All the mice were fasted, but with free access to water for 12 h and executed, and the liver and serum were obtained. The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum of the mice, and the superoxide dismutase (SOD) activity, the levels of triglyceride (TG) and malondialdehyde (MDA) in the liver tissue of the mice were determined.Results: The total content of lignan monomers in Schisandra chinensis YH was the highest, the second was Schisandra chinensis SH, and 156-5-2 and 50-1-5 were later. Except for 156-5-2, other 3 kinds of Schisandra chinensis had strong antioxidant effects, and the order of antioxidant activity was YH>SH>50-1-5. Compared with model group, the ALT and AST levels in the serum and the levels of MDA and TG in the liver tissue of the mice in YH group were decreased significantly (P<0.05 or P<0.01);the TG level in the liver tissue of the mice in SH group was reduced significantly (P<0.05), but the serum ALT and AST levels didn't change significantly(P>0.05);the ALT level in the serum of the mice in 156-5-2 group was decreased(P<0.05), but the MDA and TG levels in the liver tissue didn't change significantly(P>0.05).Conclusion: In 4 strains of Schisandra chinensis, YH has the highest content of lignans, has the strongest antioxidant effect in vitro,and has the best protective effect on acute alcoholic liver injury.

Key words: Schisandra chinensis, lignans, antioxidant, alcoholic liver injury

中图分类号: 

  • R285.5