姜黄素预处理对急性缺血再灌注模型大鼠肝脏的保护作用

doi:10.13481/j.1671-587x.20200215

摘要/Abstract

摘要： 目的：建立姜黄素预处理的大鼠急性肝脏缺血再灌注模型，初步探讨姜黄素对急性缺血再灌注后大鼠肝脏的保护作用。方法：10只SD大鼠随机分为假手术组（n=3）、溶剂对照组（n=3）和实验组（n=4）。实验组和溶剂对照组大鼠在进行急性肝脏缺血再灌注前分别腹腔注射姜黄素和1%羧甲基纤维素钠（CMC）进行预处理。假手术组大鼠不进行急性肝脏缺血再灌注。采用超高效液相色谱-质谱（UPLC-MS）联用法分析各组大鼠血清中姜黄素水平，胆管导管术和荧光分析法检测各组大鼠胆汁流量，丙二醛（MDA）试剂盒检测各组大鼠肝脏组织中MDA水平。结果：腹腔注射200mg·kg^-1姜黄素1h后大鼠血清中姜黄素水平达到（0.17±0.05）mg·L^-1，并在注射3h后降到检测线以下。实验组大鼠肝脏组织中MDA水平为（9.18±1.78）mmol·g^-1，低于溶剂对照组（527.54mmol·g^-1±237.38mmol·g^-1）和假手术组（162.73mmol·g^-1±90.50 mmol·g^-1）。实验组大鼠在缺血再灌注后胆汁流量恢复到基线的40%左右，与溶剂对照组比较差异无统计学意义（P>0.05），假手术组大鼠胆汁流量则保持在基线水平。结论：姜黄素腹腔注射给药后能迅速进入大鼠血液循环，并在体内较快代谢，其水平在给药3h后降到检测线以下。姜黄素预处理能有效降低缺血再灌注后肝脏组织中MDA水平，对肝脏起到保护作用，但对恢复胆汁流量的效果不明显。

关键词: 姜黄素, 肝脏, 缺血再灌注, 胆管导管术, 丙二醛

Abstract: Objective: To establish the acute liver ischemia-reperfusion models of rats pre-treated with curcumin, and to investigate the protective effect of curcumin on the liver of the rats after acute ischemia-reperfusion. Methods: Ten SD rats were randomly assigned to sham operation group(n=3),solvent control group(n=3) and curcumin group(n=4).The rats in curcumin group and solvent control group were pre-treated with curcumin and 1% carboxymethyl cellulose (CMC) respectively by intraperitoneal injection before acute liver ischemia-reperfusion. The rats in sham operation group did not receive acute liver ischemia-reperfusion. Ultra performance liqwid chromatography-mass specteum(UPLC-MS) was used to determine the levels of serum curcumin of the rats in various. Bile duct cannulation and flurescence analysis were used to measure the bile flows of the rats in various groups. The malondialdehyde (MDA) kit was used to detect the MDA levels in liver tissue of the rats in various groups. Results: The serum curcumin level of the rats 1 h after introperitoneal injection of 200 mg·kg^-1 curcumin reached to(0.17±0.05)mg·L^-1, but decreased to below the detection level at 3 h after injection. The MDA level in liver tissue of the rats in curcumin group (9.18mmol·g^-1±1.78mmol·g^-1) was lower than those in solvent control group (527.54mmol·g^-1±237.38mmol·g^-1) and sham operation group (162.73mmol·g^-1±90.50mmol·g^-1).The bile flow of the rats in curcumin group recovered to approximately 40% of basal flow after ischemia-reperfusion, and had no significant difference compared with solvent control group (P>0.05).The bile flow of the rats in sham operation group kept in the basal flow level. Conclusion: Curcumin could be readily detected in the blood of the rats after introperitoneal injection; it is metabolized rapidly in vivo, and its level could decrease to below the detection level at 3 h after administration. Pre-treatment with curcumin can protect the liver by decreasing the MDA level in liver tissue of the rats after liver ischemia-reperfusion. However the treatment has the limited effect on bile flow restoration.

Key words: curcumin, liver, ischemia-reperfusion, bile duct cannulation, malondialdehyde

中图分类号:

R285.5

周亚宾, 华进, 戚伶俐, 代露, 庞晓丽. 姜黄素预处理对急性缺血再灌注模型大鼠肝脏的保护作用[J]. 吉林大学学报(医学版), 2020, 46(02): 297-301.

ZHOU Yabin, HUA Jin, QI Lingli, DAI Lu, PANG Xiaoli. Protective effect of curcumin pre-treatment on liver of acute ischemia-reperfusion model rats[J]. Journal of Jilin University(Medicine Edition), 2020, 46(02): 297-301.

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