吉林大学学报(医学版) ›› 2020, Vol. 46 ›› Issue (6): 1260-1266.doi: 10.13481/j.1671-587x.20200624

• 基础研究 • 上一篇    下一篇

川陈皮素对人前列腺癌DU145细胞生长的抑制作用及其机制

张阳,姜华茂()   

  1. 锦州医科大学附属第一医院泌尿外科,辽宁 锦州 121000
  • 收稿日期:2020-05-07 出版日期:2020-11-28 发布日期:2022-08-24
  • 通讯作者: 姜华茂 E-mail:lyyyjhm@163.com
  • 作者简介:张 阳(1991-),男,辽宁省朝阳市人,在读医学硕士,主要从事泌尿系肿瘤的基础和临床方面的研究。
  • 基金资助:
    辽宁省人社厅“百千万人才工程”科研项目资助课题(辽人社〔2019〕45号)

Inhibitory effect of nobiletin on growth of human prostate cancer DU145 cells and its mechanism

Yang ZHANG,Huamao JIANG()   

  1. Department of Urology,First Affiliated Hospital,Jinzhou Medical University,Jinzhou 121000,China
  • Received:2020-05-07 Online:2020-11-28 Published:2022-08-24
  • Contact: Huamao JIANG E-mail:lyyyjhm@163.com

摘要: 目的

检测川陈皮素对人前列腺癌DU145细胞增殖、凋亡、迁移和侵袭能力的影响,探讨其作用机制。

方法

将培养的人前列腺癌DU145细胞分为对照组和0.8、1.6、3.2、6.4、12.8、25.6和51.2 μmol·L-1 川陈皮素组。MTT法检测各组人前列腺癌DU145细胞的增殖活性,细胞划痕实验分析各组人前列腺癌DU145细胞迁移能力,Transwell实验分析各组人前列腺癌DU145细胞侵袭能力,流式细胞术检测各组人前列腺癌DU145细胞凋亡率,Western blotting法检测各组人前列腺癌DU145细胞中磷酸化c-Jun氨基末端激酶(p-JNK)、磷酸化P38丝裂原活化蛋白酶(p-P38)和磷酸化细胞外调节蛋白激酶(p-ERK)蛋白表达水平。

结果

与对照组比较,作用24、48和72 h 时,25.6 μmol·L-1 川陈皮素组人前列腺癌 DU145细胞增殖活性明显降低(P<0.01),川陈皮素对DU145细胞的增殖抑制作用呈现时间和浓度依赖性。与对照组比较,作用48 h后,12.8 μmol·L-1 川陈皮素组人前列腺癌DU145细胞划痕愈合率和侵袭细胞数明显降低(P<0.01),人前列腺癌DU145细胞中p-ERK蛋白表达水平降低(P<0.01);25.6 μmol·L-1 川陈皮素组人前列腺癌DU145细胞中p-ERK蛋白表达水平降低(P<0.01),但细胞凋亡率明显升高(P<0.01),p-JNK和p-P38蛋白表达水平升高(P<0.01)。

结论

川陈皮素对人前列腺癌DU145细胞生长具有抑制作用,可促进细胞凋亡,抑制细胞增殖、迁移和侵袭;MAPK途径相关蛋白表达的改变可能是川陈皮素对DU145细胞生长抑制作用的机制之一。

关键词: 前列腺肿瘤, 人前列腺癌DU145细胞, 川陈皮素, 丝裂原活化蛋白激酶

Abstract: Objective

To detect the effects of nobiletin on proliferation, apoptosis, migration and invasion of the human prostate cancer DU145 cells, and to explore its mechanism.

Methods

The DU145 cells were cultured and divided into control and 0.8,1.6,3.2,6.4,12.8,25.6,and 51.2 μmol·L-1 nobiletin groups. MTT method was used to detect the proliferation activities of human prostate cancer DU145 cells in various groups; cell scratch test was used to analyze the cell migration abilities of human prostate cancer DU145 cells in various groups;Transwell test was used to analyze the invasion abilities of human prostate cancer DU145 cells in various groups;flow cytometry was used to detect the apoptotic rates of human prostate cancer DU145 cells in various groups. The expression levels of phosphorylated c-Jun N-terminal kinase (p-JNK), phosphorylated P38 mitogen activated protease (p-P38), and phosphorylated extracellular regulated protein kinase (p-ERK) proteins in human prostate cancer DU145 cells in various groups were analyzed by Western blotting method.

Results

Compared with control group, the proliferation activities of human prostate cancer DU145 cells in 25.6 μmol·L-1 nobiletin group after treated for 24, 48, and 72 h were significantly decreased(P<0.01); nobiletin inhibited the growth of DU145 cells in time- and concentration-dependent manners. Compared with control group, after treated for 48 h,the scratch healing rate and the number of invasion cells of human prostate cancer DU145 cells in 12.8 μmol·L-1 nobiletin group were significantly decreased(P<0.01), and the expression level of p-ERK protein was decreased (P<0.01); the expression level of p-ERK protein in human prostate cancer DU145 cells in 25.6 μmol·L-1 nobiletin group was decreased(P<0.01),the apoptotic rate was significantly increased(P<0.01), and the expression levels of p-JNK and p-P38 proteins were increased(P<0.01).

Conclusion

Nobiletin has the inhibitory effect on the growth of human prostate cancer DU145 cells and it can promote the apoptosis and inhibit the cell proliferation,migration and invasion. The changes of MAPK related protein expressions may be one of the mechanisms of the inhibitory effect of nobiletin on the growth of human prostate cancer DU145 cells.

Key words: prostate neoplasms, human prostate cancer DU145 cells, nobiletin, mitogen-activated protein kinase

中图分类号: 

  • R737.25