吉林大学学报(医学版)

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TPX2 和Aurora A 在口腔鳞癌组织中的表达及其临床意义

武 燃1，李明贺1，左伟文2，高 尚3，张茹慧1   

  1. 1.吉林大学口腔医院口腔颌面外科， 吉林 长春 130021；2.吉林大学口腔医院口腔修
    复科，吉林 长春 130021；3.吉林大学口腔医院正畸科， 吉林 长春 130021
  • 收稿日期:2013-04-01 出版日期:2013-07-28 发布日期:2013-08-17
  • 通讯作者: 张茹慧（Tel：0431-85579361，E-mail: mingming9628@sina.com） E-mail:mingming9628@sina.com
  • 作者简介:武 燃（1987-），男，河北省唐山市人，在读医学硕士，主要从事口腔颌面部 肿瘤基础与临床方面的研究。
  • 基金资助:

     吉林省科技厅科研基金资助课题（20100134）

Expressions of TPX2 and Aurora A in oral squamous cell 
carcinoma tissue and their clinical significances


WU Ran1，LI Ming-he1，ZUO Wei-wen2，GAO Shang3，ZHANG Ru-hui1   

  1. (1.Department of Oral and Maxillofacial Surgery,Stomatology Hospital,Jilin University,Changchun 130021,China;2.Department of Prosthodontics,Stomatology Hospital,Jilin University,Changchun 130021,China;3.Department of Orthodontics,Stomatology Hospital,Jilin University,Changc
    hun 130021,China)
  • Received:2013-04-01 Online:2013-07-28 Published:2013-08-17

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摘要：

目的:观察靶向Xklp2靶蛋白(TPX2) 和Aurora A在口腔鳞癌组织中的表达，探讨TPX2 和Aurora A表达在口腔鳞癌治疗和预后中的意义。方法:选择口腔鳞癌标本61例，另选29 例癌旁组织和61 例正常口腔黏膜组织为对照组。应用免疫组织化学SP法检测TPX2 和Aurora A蛋白的阳性表达，在显微镜下观察阳性细胞所占百分比，并进行结果判定。采用Spearman等级相关分析法分析TPX2 和Aurora A的蛋白表达相关性。结果:免疫组织化学染色,TPX2 蛋白在正常黏膜组织、癌旁组织和口腔鳞癌组织的阳性表达率依次增高,分别为4.9 %(3/61)、51.7%(15/29) 和86.9%(53/61),各组间比较差异均有统计学意义( P<0.05)；Aurora A 蛋白在正常黏膜组织、癌旁组织和口腔鳞癌组织的表达率依次增高,分别为3.3%(2/61)、44.8 %(13/ 29) 和72.1 %(44/ 61),各组间比较差异均有统计学意义( P<0.05)。TPX2 的蛋白表达水平与口腔鳞癌的淋巴结转移有密切关联(P<0.05)。随Aurora A表达水平的增高，口腔鳞癌的恶性程度和淋巴结转移的可能性也相对增加 ( P<0. 05)。Spearman等级相关分析，在口腔鳞癌组织中TPX2与Aurora A蛋白的表达呈正相关关系（r= 0.445，P<0.01）。结论:TPX2和Aurora A在口腔鳞癌的发生和转移过程中均具有重要的促进作用，且二者间也有相互促进的关系。

关键词: 口腔鳞状细胞癌, 免疫组织化学, 靶向Xklp2靶蛋白

Abstract:

Objective To investigate the expressions of TPX2 and Aurora A  in oral squamous cell carcinoma(OSCC)tissue and explore the significances of TPX2 and Aurora A expressions in the  treatment and prognosis of oral squamous cell carcinoma.Methods 61 cases of oral squamous cell carcinoma were chosen as experimental group, other 29cases of adjacent tissue of cancer and 61 cases of normal oral mucosa tissue were chosen as control group.　Immunohistochemical staining  was used to detect  the positive expressions  of TPX2 and Aurora A.The percentages of positive cells were observed under  microscope.The correlation between TPX2 and Aurora A protein expressions was analyzed with Spearman analysis.Results The results of immunohistochemical staining showed that the positive expression rates of TPX2 protein in normal mucosa tissue,adjacent tissue of cancer and cancer tissue were 4.9% (3/ 61),51.7% (15/29),and 86.9% (53/61);and there were statistically significant difference among all groups (P<0.05).The expression of TPX2 protein was closely related to the lymph node metastasis of oral squamous cell carcinoma (P<0.05).The positive expression rates of Aurora A protein in normal mucosa tissue,adjacent tissue of cancer and cancer tissue were  3.3% (2/ 61),44.8% (13/29),and 72.1% (44/61);and there were statistically significant difference among all groups (P<0.05).And with the increasing of Aurora A expression level,the malignant  degrees and possibility of lymph node metastasis in oral squamous cell carcinomas were also increased,which had statistically significant difference among groups (P<0.05).The TPX2 protein expression in oral squamous cell carcinoma tissue had positive correlation with the Aurora A protein expression(r=0.445，P<0.01).Conclusion TPX2 and Aurora A may play important roles in promoting the infiltration and metastasis of mucosal epithelium in oral squamous cell carcinoma,and the relationship between the two genes is collabortion and mutual promotion.

Key words: oral squamous cell carcinoma, immunohis
tochemistry, targeting protein for Xenopus kinesin-like protein 2

中图分类号: 

  • R739.8
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