关键词: 癌, 肝细胞, 细胞周期

Abstract: Objective To study the effect of LaCl₃ on the growth of CBRH-7919 cells. Methods The growth, clone inhibitory rate and general histological changes of CBRH-7919 cells were observed at different time (1th,3rd and 5th day) after treated with different doses (0.01,0.10 and 1.00 mmol•L^-1) of LaCl₃ in vitro. At the same time, the changes of cell cycle were detected by flow cytometry. Results The evident inhibition of LaCl₃ to CBRH-7919 cells growth occurred at 3rd day after treatment (P<0.01), gradually strengthened following the increase of dose and time (P<0.01). The result of clone formation assay expressed that the clone formations of CBRH-7919 cells were inhibited distinctly at the concentration of 0.10 and 1.00 mmol•L^-1 LaCl₃ (P<0.01). The result indicated that treated by LaCl₃, the distribution of cell cycle changed as followed: the cell percentage of G₀/G₁ period was increased, the ratios of groups of 0.01, 0.1 and 1.00 mmol•L^-1 LaCl₃ were evidently increased compared with the control and presented density-dependent relation (P<0.01); The cell percentage of S period was reduced, the rations of groups of 0.10 and 1.00 mmol•L^-1 LaCl₃ were significantly reduced compared with the matched control (P<0.05). Conclusion LaCl₃ can inhibit the growth of CBRH-7919 cells obviously.

Key words: carcinoma, hepatocellular, cell cycle