关键词: 髓性, 慢性, 诊断, 聚合酶链反应, bcr, abl, 原癌基因

Abstract: Objective To increase the sensitivity of residual leukemic cells detection in chronic myelocytic leukemia (CML) patients with RT-PCR, the optimal dose of reverse transcriptase，optimal annealing，extension temperature，and PCR cycles were studied to confirm bcr/abl fused gene types. Methods Through changing the PCR conditions, the dose of reverse transcriptase was decreased to one-quarter of routine doses (5 U),the annealing temperature was increased from 50℃ to 60℃, and the number of reaction cycles was increased from 30 to 45. All 164 CML patients were examined. Results bcr/abl fused gene types were found in 155 patients with positive Ph chromosome,of all 78 cases were b₃/a₂ type, 54 cases were b₂/a₂ type, and 23 cases were both b₃/a₂ and b₂/a₂ types. Of 9 negative Ph chromosome, 1 case was b₃/a₂ type, 3 cases was b₂/a₂ type, 1 case was both b₃/a₂ and b₂/a₂ types,and 4 cases were negative (Ph^-bcr^-CML). Conclusion Almost all the CML patients can be found that they have bcr/abl fused gene, few have two kinds of chimera leukemic cells (two kinds of mutational cell lines).

Key words: myeloid, chronic, diagnosis, polymerase chain reaction, bcr, abl, proto-oncogenes