关键词: 细胞学, 细胞, 培养的, 干细胞, 造血系统, 辐射, 电离

Abstract: Objective To apply skin mesenchymal stem cells (MSCs) irradiated with different dosages of X-rays, instead of MSCs in the bone marrow to promote the proliferation of hematopoietic stem cells in vitro. Methods The bone marrow cells were cultured on the skin MSCs layer irradiated with different dosages of X-rays in vitro. The number of bone marrow cells, the colony-forming-unit-macrophage-granulocyte (CFU-GM) were assayed after expansion and maintenance for 0-12 days. CFU-GM, colony-forming unit-erthroid（CFU-E）, burst-forming-unit-erythroid （BFU-E）colony were assayed in the cultured supernatant of the skin MSCs. The effects of supporting hematopoiesis of irradiation on skin MSCs in fetal mice were observed. Results The CFU-GM colonies were increased after bone marrow cells were cultured together with fetal mice skin MSCs irradiated with 6,8, and 10 Gy X-rays, especially in 6 Gy X-rays group, it was 2.17 times as big as that in 0 Gy X-rays group. The cultured supernatant of skin MSCs irradiated with 6,8 and 10 Gy X-rays could stimulate the growth of CFU-GM, CFU-E, and BFU-E in vitro, there were significant differences compared with those in 0 Gy X-rays group (P<0.05), especially in 6 Gy X-rays group, they were 1.81,3.94,and 3.51 times as big as those in 0 Gy X-rays group,respectively.Conclusion Skin MSCs irradiated with certain dosage of X-rays in vitro have the function of sustaining the proliferation of hematopoietic cells,both irradiated MSCs layer and supernatant can increase the number of bone marrow cells colony.

Key words: cytology, cells, cultured, stem cells, hematopoietic system, radiation, ionizing