关键词: 前列腺肿瘤, 基质金属蛋白酶抑制剂-3, 基质金属蛋白酶, 肿瘤细胞, 培养的

Abstract: Objective To assess the effects of tissue inhibitor of metalloproteinase-3 (TIMP-3) on proliferation and invasion of PC-3M cell line in vitro. Methods The adhesion assay was used to detect the effects of TIMP-3 on cancer cell adhesion abilities. MTT colorimetry was used to detect the proliferation abilities of PC-3M cells. Monolayer and Boyden chamber invasion assay were performed to assess cell invasion abilities. Results The adhesion rate and proliferation rate of transfected cells containing TIMP-3 gene were significantly lower than those of cells transfected with empty plasmid and untransfected cells (P<0.05). The cell invasion number and index of the former were significantly lower than the others (P<0.05). The differences of adhesion rate, proliferation rate and invasion index between transfected empty plasmid cells and untransfected cells weren′t statistically significant (P>0.05). Conclusion TIMP-3 can significantly inhibit the proliferation and invasion of human prostate carcinoma PC-3M cell line.

Key words: prostatic neoplasms, tissue inhibitor of metalloproteinase-3, matrix metalloproteinases, tumor cells, cultured