关键词: 丝裂霉素, 药理学, 氟尿嘧啶, 药理学, 顺铂, 药理学, 细胞周期, 药物筛选试验, 抗肿瘤, 紫杉醇

Abstract: Objective To observe the effects of paclitaxel, mitomycin, 5-FU and cisplatin on the proliferation of tongue carcinoma cells and to guide the reasonable using of chemotherapeutic drugs and avoids the side-effects. Methods The fresh tongue carcinoma tissues from surgery were collected and unicellular suspension were prepared. Then paclitaxel, mitomycin, 5-FU and cisplatin were used to investigate the drug sensitivity on primary tongue carcinoma cells and a blank control group was set up. The inhibitory effects of chemotherapeutic drugs on the growth of tongue carcinoma cells and the cell cycle phases were observed by MTT colorimetry and flow cytometry. Results The inhibitory rates of paclitaxel, mitomycin and 5-FU on tongue carcinoma cell proliferation were 45.3%, 37.3%, and 36.2%, respectively, and in control group it was 2.1%, the difference was significant（P< 0.01）. But there was no significant different of inhibitory rate between cisplatin and control group. The results of flow cytometry showed that the amount of cells increased in G₀/G₁ phase, decreased in S phase and relatively increased in G₂/M phase after using paclitaxel, mitomycin and 5-FU compared with control group（P<0.01）. 　 Conclusion Paclitaxel, mitomycin and 5-FU can inhibit the growth of tongue carcinoma cells distinctively and should be considered prior to other drugs.

Key words: mitomycin, pharmacology, fluorouracil, pharmacology, cisplatin, pharmacology, cell cycle, drug screening assays, antitumor, taxol