关键词: runx3, 基因, P53, DNA甲基化, 突变

Abstract: Objective To observe the mechanism of the change in runx3 in gastric carcinoma itsdifference and with p53 mutation. Methods A total of 30 cases of gastric carcinoma were taken from clinical operation. PCR-SSCP method was used to detect mutations in exons 2，3，4 of runx3 gene in the gastric carcinoma.The methylation status of runx3 gene was examined by methylation-specificpolymerase chain reaction (MS-PCR), PCR-SSCP method was used to detect mutations in exons 5，6，7，8 of p53 gene in the gastric carcinoma. Results Two cases with mutation were found ,methylation of runx3 promoter region was confirmed in 87% (26/30) specimens of gastric carcinoma, 53.3％（16/30）cases were confirmed with mutati on in the p53 gene. The rate of methylation of runx3 was higher than the rate of mutation of p53（P<0.05）.Conclusion The gene mutation of runx3 is not the change of the molecular biology of gene in the gastriccarcinoma.High frequent methylation in the promoter region of runx3 is primarychange in the gastric carcinoma; compared with the mutation in the p53 gene, high frequent methylation in the promoter region of runx3 has genetic specificity and runx3 is pivotal gene in the development of gastric carcinoma.

Key words: runx3, gene p53, DNA methylation, mutation