关键词: 粒细胞-巨噬细胞集落刺激因子, 黑色素瘤, 实验性, 基因疗法

Abstract: Objective To investigate the anti-tumor effects of combined recombinant murine interleukin-12 and GM-CSF gene adenovirus vector (AdCMVIL-12, AdCMVGM-CSF) on B16 melanoma. Methods B16 melanoma cells were injected subcutaneous at right back of C57/BL6 mice, 48 tumor bearing mice were divided into 4 groups at random, when tumor diameter attained to 5 mm, AdCMVIL-12,AdCMVGM-CSF and AdCMVIL-12+AdCMVGM -CSF and AdCMVLacZ (control) were injected in different groups, tumor growth and immune responses of each group were tested, and the IL-12 and GM-CSF levels in serum were observed. Results In combined group, necrosis, lymphocytes and macrophages were found in tumor tissues. 30 d after treatment, the average volume in combined group was （60.73±11.36）mm³, compared with AdCMVLacZ (control) group, and AdCMVIL-12 group, AdCMVGM-CSF group ［(675.18±80.59）,（186.91±19.15）,（223.45±23.11）mm³］, there were significant differences (P<0.01). Combined IL-12 and GM-CSF gene therapy could enhanced　anti-tumor immune response of B16 melanoma bearing mice and increased the expressing time of IL-12 and GM-CSF, and had very strong specific kill effect. Kill probability was （69.53±9.20）%. Conclusion Combined IL-12 and GM-CSF gene therapy can enhance the anti-tumor immune response of B16 melanoma bearing mice and decrease side -effect of using AdCMVIL-12.

Key words: granulocyte-macrophage colony-timulating factor, melanoma, experimental, gene therapy