关键词: 雌激素, 中脑导水管周围灰质, 5-羟色胺, 行为学

Abstract: Abstract:Objective To observe the effects of estrogen on behavior and 5-HT in periaqueductal gray （PAG）in migraine model rats. Methods Tewnty-four ovariectomized Wistar rats were divided randomly into four groups：control group (Group A)，migraine group(Group B)，low dose estradiol-treated ovariectomized group(Group C)， high dose etradiol-treated ovariectomized group(Group D).After 1 week，the rats in Group B,C and D were injected with nitroglycerine 10 mg•kg^-1 ubcutaneously to make migraine rat models, the rats in Group A were given peanut oil alike，and the behavior changes were observed.2 h after injection,the rats were killed and the midbrains were separated and then 5-HT immunohistochemical staining was performed.Results Behavior: compared with Group B，the degrees of red-calws,red-ears and red-tail rats in Group D relieved obviously，the times of climbing hutch and scratching head were much fewer, while the rats in group C showed no significant difference;Immunohistochemical staining:compared with Group A，the 5-HT-positive neurons expression in PAG of Group B and C were more obviously（P<0.01）, especially in the ventrolateral and dorsolateral of PAG,the degree of 5-HT-positive neurons increased in PAG of Group B as most,Group C as more,while group D as a little. There were significant differences between various groups(P<0.01). Conclusion Estrogen can reduce the degree of 5-HT-positive neurons activating in PAG,and its role in a dose-dependent manner,and high dose estrogen can decrease the changes of behavior of migraine rats, the mechanism may be related with that estrogen can influence the neuroactive substances taking part in migraine.

Key words: estrogen, periaqueductal gray, 5-hydroxytryptamime, behavior