aurora-A基因在卵巢癌组织中的表达及其临床病理意义

doi:吉林省科技厅基金资助课题（2007005144）

aurora-A基因在卵巢癌组织中的表达及其临床病理意义

牛春波¹,贾飞勇²,岳英³,闫旭¹,曲丽梅¹

1.吉林大学第一医院病理科，吉林长春 130021；2.吉林大学第一医院儿科，吉林长春 130021；3.吉林大学第一医院妇产科，吉林长春 130021

收稿日期:2007-10-31 修回日期:1900-01-01 出版日期:2008-07-28 发布日期:2008-07-28
通讯作者: 贾飞勇

Expression of aurora-A gene in ovarian cancer tissue and its clinicopathologic significance

NIU Chun-bo¹,JIA Fei-yong², YUE Ying³,YAN Xu¹,QU Li-mei¹

1.Department of Pathology,First Hospital，Jilin University,Changchun 130021,China;2. Department of Pediatrics,First Hospital，Jilin University,Changchun

Received:2007-10-31 Revised:1900-01-01 Online:2008-07-28 Published:2008-07-28
Contact: JIA Fei-yong

摘要/Abstract

摘要： 目的：检测卵巢癌aurora-A基因扩增和蛋白表达特性，探讨aurora-A基因在卵巢癌发生发展及转归过程中的作用。方法：应用定量RT-PCR法检测aurora-A基因在6例正常卵巢及8例卵巢癌组织中的扩增情况，用免疫组化法检测aurora-A蛋白在6例正常卵巢及40例卵巢癌组织中的表达，并分析卵巢癌aurora-A蛋白过度表达与其病理分型、组织分化、临床分期、肿瘤增殖活性和预后之间的关联性。结果：定量RT-PCR检测表明，与正常卵巢对比，8例卵巢癌aurora-A基因的mRNA表达量明显增高（P<0.005）；免疫组化结果显示，aurora-A蛋白在6例正常卵巢组织中均呈阴性表达，在23例（57.5%）卵巢癌组织中呈阳性表达，且aurora-A蛋白表达增高与卵巢癌临床分期、肿瘤增殖活性及预后有关联性（P<0.05），而与卵巢癌的病理分型和组织分化无明显关联性（P>0.05）。结论：卵巢癌组织存在aurora-A基因异常扩增和蛋白过度表达，aurora-A可能在卵巢癌的发生发展过程中起着重要的促进作用，针对aurora-A基因及蛋白的生物治疗有可能成为肿瘤治疗的有效途径。［关键词］［中图分类号］［文献标识码］ A Expression of aurora-A gene in ovarian cancer tissue and itsclinicopathologic signif () Abstract:

关键词: aurora-A基因, 聚合酶链反应

Abstract: Objective To detect the genetic amplification and protein expression characteristics of aurora-A in ovarian cancer and to interpret the role of aurora-A gene in course of onset,progression and regression phases of ovarian cancer. Methods The amplification of aurora-A gene was detected by quantitative PCR in 6 normal ovarian tissues and 8 ovarian cancer samples,and its protein expression was examined by immunohistochemistry in 6 normal ovarian tissues and 40 ovarian cancer samples,furthermore,the relationships between over-expression of aurora-A protein in ovarian cancer tissue and its pathologic classification,tissue differentiation,clinical phase,tumor proliferation trait and prognosis were analyzed. Results Quantitive PCR showed that aurora-A mRNA was significantly higher in 8 ovarian cancer samples than that in normal ovarian tissues（P<0.005）;Immunohistochemical assay showed that negative expression was in 6 normal ovarian tissues,whereas 57.5% was positive expression in ovarian cancer samples,and the evelation of aurora-A protein expression had a correlationship with clinical phases,tumor proliferati on and prognosis in ovarian cancer(P<0.05）,however, there was no correlationship between aurora-A protein expression and pathological classification and tissue differentiation（P>0.05）. Conclusion There are abnormal amplification and protein over-expression of aurora-A in ovarian cancer tissue,aurora-A probably play an important role in the onset and progression of ovarian cancer,and the novel biological treatment concerning aurora-A gene and its protein is probably a useful route for curing tumor.

Key words: aurora-A, polymerase chain reaction

中图分类号:

R737.31

牛春波,贾飞勇,岳英,闫旭,曲丽梅. aurora-A基因在卵巢癌组织中的表达及其临床病理意义[J]. J4, 2008, 34(4): 653-656.

NIU Chun-bo,JIA Fei-yong, YUE Ying,YAN Xu,QU Li-mei. Expression of aurora-A gene in ovarian cancer tissue and its clinicopathologic significance[J]. J4, 2008, 34(4): 653-656.

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