关键词: 乙型, 慢性, 肝硬化, 病毒复制, 白细胞介素12, 白细胞介素18

Abstract: To investigate the effect and clinical significance of HBV replication on the serum levels of IL-12 and IL-18 in patients with hepatitis B-related cirrhosis.Methods The levels of HBV-DNA and IL-12 and IL-18 in peripheral blood from 136 patients with different clinic types chronic hepatic diseases（102 patients with chronic hepatitis B,34 patients with hepatitis B-related cirrhosis ），95 HBV carriers and 20 healthy people (as control group) were respectively detected with ELISA and real time PCR. Results The levels of IL-12 and IL-18 in chronic hepatitis B and hepatitis B-related cirrhosis groups were significantly increased compared with control group(P<0.05). The levels of IL-12 and IL-18 in high replication groups of serum HBV-DNA(HBV≥10⁵•mL^-1) were markedly higher than those in negative HBV-DNA group(HBV-DNA＜10³•mL^-1,P<0.05)；there was no significant difference of the serum IL-12 and IL-18 levels between chronic hepatitis B group and hepatitis B-related cirrhosis group，and among different HBV carriers（P>0.05）; and there was positive relationship between prevalence of HBV replication and IL-12,IL-18 levels　in different chronic hepatic disease groups（r=0.74,P<0.05;r=0.80,P<0.05）. Conclusion HBV replication can affect the levels of IL-12 and IL-18 in peripheral blood in patients with hepatitis B，patients with hepatitis and B-related cirrhosis, the higher the levels of the HBV replication,the higher the levels of IL-12 andI L-18 in different chronic hepatic diseases.

Key words: chronic, liver cirrhosis, virus replication, interleakin-12, interleukin-18