关键词: 电离, 巨噬细胞, Toll样受体, 髓样分化因子88

Abstract: Abstract:Objective To observe the effect of ionizing radiation on the expressions of TLR4 and adaptor protein MyD88 in mouse peritoneal macrophages in the Toll-mediated signal transduction pathways. Methods 160 male Kunming white mice were randomly divided into 16 groups ( ten each group )：sham-irradiation and five groups after 4,8,16,24 and 48 h X-ray irradiation for time-course experiments;sham-irradiation and nine groups after 0.05,0.075,0.100,0.200,0.500,1.000,2.000,4.000,6.000 Gy irradiation for dose-effect experiments. Flow cytometry was used to detect the expressions of TLR4 and MyD88 after whole body irradiation (WBI) with X-rays. Results Both of TLR4 and Myd88 expressed more than sham-irradiation after 0.075 and 2.000 Gy WBI for 4 h，and the expressions of them reached the peak at 16 h or 4 h after WBI(P<0.05 or P<0.01) in time-course experiments. In dose-effect experiments,the expression levels of TLR4 and MyD88 increased gradually with the enhancement of X-rays irradiation dose，both of them reached the peak at 2.000 Gy WBI,and there was significant difference compared with sham-irradiation group (P<0.01). Conclusion X-ray irradiation can increase the expressions of TLR4 and MyD88 in mouse peritoneal macrophages，promote the immune respose and start the adaptive immunity of mice.

Key words: ionizing, macrophages, Toll-like receptor 4, myeloid differetiation factor 88