关键词: prostasin, 免疫组织化学, 逆转录聚合酶链反应

Abstract: Abstract:Objective To study the expression of prostasin in ovarian serous cystadenocarcinoma tissues and disscuss the significances of prostasin gene in the occurrence and development of ovarican carcinoma.Methods RT-PCR method was used to investigate the expressions of prostasin mRNA in tissue samples of 10 ovarian serous cystadenocarcinoma,10 ovarian serous cystadenoma and 10 normal ovary.The expressions of prostasin protein in 60 ovarian serous cystadenocarcinoma,24 ovarian serous cystadenoma and 24 normol ovarian tissues were detected by immunohistochemistry. Results The level of prostasin mRNA expression in ovarian serous cystadenocarcinoma tissues（0.415±0.177）was significantly higher than those in normal ovarian tissues（0.192±0.128）and ovarian serous cystadenoma tissues（0.237±0.232）（P<0.05）.The expression of prostasin protein was mostly located in cytoplasm;the positive expression rate of prostasin protein in ovarian serous cystadenocarcinoma group was 65.0%,which was significantly higher than those in normal ovarian group(12.5%)and ovarian serous cystadenoma group(25.0%) (P<0.01);there was correlation between the expression intensity of prostasin protein and pathological grading and clinical stages;the higher clinical stage and the worse differentiation of ovarian serous cystadenocarcinoma,the stronger positive expression of prostasin protein. Conclusion Prostasin gene plays a promotion effect on the course of occurrence and development of ovarian carcinoma.

Key words: prostasin, immunohistochemistry, reverse transcriptase polymerase chain reaction