关键词: 乳腺肿瘤, 受体, 雌激素, 蛋白质组, 二维电泳, 质谱

Abstract: Objective To explore potential biomarker candidate(s) in stage Ⅱ breast infiltrating ductal carcinoma with negative estrogen receptor (ER^-) and to discuss the significance of the identified proteins in tumor invasion and metastasis.Methods Two-dimensional polyacrylamide gel electrophoresis (2-DE) in combination with matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-MS) and bioinformatics analysis were performed to investigate differential proteins of the infiltrating ductal carcinoma at stage Ⅱand adjacent normal breast tissues from 5 breast cancer patients with negative estrogen receptor.Results The number of the tested protein spots from 5 adjacent normal breast tissues was from 779 to 1 328.The number of the tested protein spots from 5 breast cancer tissues was from 988 to 1 453.The correspondent spots was from 310 to 774. Three differential proteins (S1,S2,S3) from the 2-DE gel images of 5 patients’cancer tissues and adjacent normal tissues were obtained.Three protein candidates were identified: immunoglobulin lambda chain variable region (S1),collagen alpha-3 (Ⅵ) chain precursor (S2) and S100 calcium binding protein A11 (S3).Proteins S2 and S3 were found to be up-regulated in all 5 cancer tissues (abundance changed more than 2 folds).Conclusion Proteins can be obtained efficiently from tissues under the condition of optimized protocols of tissue 2D-PAGE;Collagen alpha-3 (Ⅵ) and S100 calcium binding protein are likely involved in invasion and metastasis in the stage Ⅱ breast infiltrating ductal carcinoma with negative estrogen receptor.

Key words: breast neoplasms, receptors, estrogen, proteome, 2-DE, MS