异氟醚预处理对缺血再灌注损伤后幼兔心肌TNF-α和iNOS mRNA表达的影响及其机制

J4 ›› 2011, Vol. 37 ›› Issue (4): 622-625.

异氟醚预处理对缺血再灌注损伤后幼兔心肌TNF-α和iNOS mRNA表达的影响及其机制

刘国利, 沈途, 张锦英

（辽宁医学院附属第一医院麻醉科|辽宁锦州121001）

收稿日期:2011-03-22 出版日期:2011-07-28 发布日期:2011-07-28
通讯作者: 沈途 E-mail:（Tel：0416-4197484，E-mail：st.1985.ling@163.com)
作者简介:刘国利（1960-）|男|辽宁省锦州市人|主任医师|硕士生导师|主要从事脏器保护的研究。
基金资助:
辽宁省教育厅科研基金资助课题（2008400）

Effects of isoflurane preconditioning on TNF-&alpha|and iNOS mRNA expressions in ischemia reperfusion myocardium of young rabbits and mechanisms

LIU Guo-Li, SHEN Tu, ZHANG Jin-Ying

（Department of Anesthesiology,First |Affiliated Hospital, Liaoning Medical College,Jinzhou 21001,China)

Received:2011-03-22 Online:2011-07-28 Published:2011-07-28

摘要/Abstract

摘要：

的：观察异氟醚(ISO)预处理对幼兔缺血再灌注心肌组织中肿瘤坏死因子-α（TNF-α）和诱导型一氧化氮合酶（iNOS） mRNA表达的影响，并阐明其作用机制。方法：取纯种新西兰幼兔36只，随机分为3组：假手术组只穿线不结扎；缺血再灌注(I/R)组，结扎30 min，再灌注2 h；ISO预处理组，缺血前吸入1.1%异氟醚30 min，洗脱15 min后处理同I/R组。缺血30 min再灌注2　h后，用放射免疫法检测心肌组织中TNF-α的含量和原位杂交法检测心肌组织中iNOS mRNA表达。结果:ISO组和I/R组幼兔心肌TNF-α含量升高，与假手术组比较差异有统计学意义(P<0.05)；而ISO组与I/R组比较，心肌TNF-α含量降低，差异有统计学意义(P<0.05)。ISO组和I/R组心肌组织中iNOS mRNA的表达水平明显高于假手术组(P<0.05)；ISO组与I/R组比较，iNOS mRNA表达水平明显下降(P<0.05)。结论：异氟醚通过减少TNF-α的生成和iNOS mRNA的表达以减轻I/R损伤而发挥保护作用。

关键词: 异氟醚；缺血再灌注；肿瘤坏死因子-α；诱导型一氧化氮合酶

Abstract:

Abstract:Objective
To investigate the effects of isoflurane(ISO) preconditioning on the expressions of TNF-α and iNOS mRNA in ischemia reperfusion myocardium of young rabbits and clarify their mechanisms.Methods 36 young rabbits were randomly divided into three groups,the rabbits in sham operation group were not occluded coronary artery;the rabbits in ischemia-reperfusion (I/R) group were occluded for 30 min and reperfused for 2 h;the rabbits in ISO preconditioning group were inhaled 1.1% isoflurane for 30 min before ischemia,others were the same to I/R group after 15 min.The content of myocardial TNF-α and the expression of iNOS mRNA in myocardioum were determined 30 min after coronary occlusion and 2 h after reperfusion. Results Compared with sham operation group,the contents of myocardial TNF-α in I/R and ISO groups were significantly increased(P<0.05);the content of myocardial TNF-α in ISO group was significantly lower than that in I/R group(P<0.05).The expressions of iNOS mRNA in I/R and ISO groups were significantly higher than that in sham operation group(P<0.05),the expression of iNOS mRNA in ISO group was significantly lower than that in I/R group(P<0.05).Conclusion ISO could reduce the TNF-α content and the expression of iNOS mRNA during I/R injury,which may be one of molecular mechanisms of ISO preconditioning in myocardial protection.

Key words: ;ischemia-reperfusion;tumor nectosis factor α;induced nitric oxide synthase

中图分类号:

R614.2

刘国利, 沈途, 张锦英. 异氟醚预处理对缺血再灌注损伤后幼兔心肌TNF-α和iNOS mRNA表达的影响及其机制[J]. J4, 2011, 37(4): 622-625.

LIU Guo-Li, SHEN Tu, ZHANG Jin-Ying. Effects of isoflurane preconditioning on TNF-&alpha|and iNOS mRNA expressions in ischemia reperfusion myocardium of young rabbits and mechanisms[J]. J4, 2011, 37(4): 622-625.