小白菊内酯对涎腺腺样囊性癌细胞系SACC-83的增殖抑制及其凋亡诱导作用

J4 ›› 2011, Vol. 37 ›› Issue (6): 1075-1078.

小白菊内酯对涎腺腺样囊性癌细胞系SACC-83的增殖抑制及其凋亡诱导作用

贾妮|张斌|黄汉|寇玉霞|董宵婷|谷小玉

辽宁医学院附属第一医院口腔科|辽宁锦州 |121001

收稿日期:2011-06-21 出版日期:2011-11-28 发布日期:2011-11-28
通讯作者: 张斌(Tel:0416-4197029,E-mail:zhangbinjin@yahoo.com.cn) E-mail:zhangbinjin@yahoo.com.cn
作者简介:贾妮（1985-）|女|山西省长治市人|在读医学硕士|主要从事涎腺恶性肿瘤侵袭的研究。
基金资助:
辽宁省教育厅高等学校科学研究项目资助课题（L2010315）；辽宁医学院博士科研启动基金资助课题（2009）

Proliferation inhibition and apoptosis induction effects of parthenolide on SACC-83 cells in vitro

Department of Stomatology|First Affiliated Hospital|Liaoning Medical College|Jinzhou 121001|China

Received:2011-06-21 Online:2011-11-28 Published:2011-11-28

摘要/Abstract

摘要：

目的：探讨小白菊内酯（Par）对涎腺腺样囊性癌细胞系SACC-83的增殖抑制作用，并阐明其相关作用机制。方法: 不同浓度(5.0、7.5、15.0、30.0、40.0和80.0 mg?L-1)Par作用于体外培养的SACC-83细胞系，同时设空白对照组；MTT比色法观察Par对SACC-83细胞系的生长抑制情况；流式细胞仪观察AnnexinV-FITC/PI双染后细胞凋亡状况；Western blotting法检测Livin蛋白的表达情况。结果: 不同浓度Par组SACC-83细胞生长受到明显的抑制，与空白对照组比较增殖抑制率明显增加，呈浓度和时间依赖性；经7.5、15.0和30.0 mg?L-1 Par作用24 h后，各组细胞凋亡率分别为14.7%±2.1%、30.5%±3.4%和38.7%±2.7%，与空白对照组凋亡率（3.2%±0.9%）比较，差异具有统计学意义（P<0.01）；不同浓度Par组Livin蛋白的表达明显下调，与空白对照组比较差异有统计学意义(P<0.05)，且各浓度组之间比较差异有统计学意义（P<0.01）。结论: Par对SACC-83细胞系有显著的增殖抑制作用，Livin蛋白的表达可能与其作用机制有关。

关键词: 小白菊内酯；涎腺肿瘤；细胞凋亡；Livin蛋白

Abstract:

Objective
To investigate the inhibitory effect of proliferation of parthenolide(Par) on salivary gland adenoid cystic carcinoma cell line SACC-83 cells in vitro and clarify its related mechanism.Methods The SACC-83 cells were treated with different concentrations(5.0，7.5，15.0，30.0，40.0 and 80.0 mg?L-1) of Par in vitro,the proliferation inhibition of SACC-83 cells was detected by MTT colorimetry,the cell cycle distribution and apoptosis status were analyzed by flow cytometry,the expression of Livin protein was determined by Western blotting.Results The growth of SACC-83 cells in Par groups was inhibited effectively in concentration-　and time-dependent manner.The apoptotic rates in Par groups were 14.7%±2.1%,30.5%±3.4% and 38.7%±2.7%, respectively, after treated with 7.5,15.0 and 30.0 mg?L-1 Par for 24 h, there were significant differences compared with control group (3.2%±0.9%,all P<0.01).The expressions of Livin proteins in Par groups were significantly down-regulated,there were significant differences compared with control group(P<0.05);and there were significant differences between different doses of Par groups（P<0.01）.Conclusion Par could obviously inhibit the proliferation of SACC-83 cells.The expression of Livin protein may be related to this effect.

Key words: parthenolide；salivary gland neoplasms；apoptosis；livin protein

中图分类号:

R739.87

贾妮|张斌|黄汉|寇玉霞|董宵婷|谷小玉. 小白菊内酯对涎腺腺样囊性癌细胞系SACC-83的增殖抑制及其凋亡诱导作用[J]. J4, 2011, 37(6): 1075-1078.