丹参磷脂浸膏对大鼠脑缺血再灌注损伤的保护作用及其机制

doi:10.13481/j.1671-587x.20150216

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (02): 291-294.doi: 10.13481/j.1671-587x.20150216

丹参磷脂浸膏对大鼠脑缺血再灌注损伤的保护作用及其机制

曲圆¹, 付丹阳^1,2, 纪影实²

1. 吉林大学第二医院骨科, 吉林长春 130041;
2. 吉林大学基础医学院药理学系, 吉林长春 130021

收稿日期:2014-10-29 出版日期:2015-03-28 发布日期:2015-04-04
通讯作者: 纪影实, 副教授, 硕士研究生导师(Tel:0431-85619799, E-mail:jiyingshi@126.com) E-mail:jiyingshi@126.com
作者简介:曲圆(1989-), 吉林省长春市人, 在读医学硕士, 主要从事股骨头缺血坏死的药物治疗方面的研究。
基金资助:
吉林省科技厅创新型企业科技项目资助课题(20140310001)

Protective effect of Danshenlinzhi on cerebral ischemia reperfusion injury in rats and its mechanism

QU Yuan¹, FU Danyang^1,2, JI Yingshi²

1. Department of Orthopedics, Second Hospital, Jilin University, Changchun 130041, China;
2. Department of Pharmacology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China

Received:2014-10-29 Online:2015-03-28 Published:2015-04-04

摘要/Abstract

摘要：

目的:观察丹参磷脂浸膏(DSLZ)对脑缺血再灌注损伤的保护作用,探讨其可能机制。方法:60只Wistar大鼠随机分为假手术组、模型组、阳性对照丹参酮ⅡA组和低、中、高剂量DSLZ组(5.0、10.0、20.0 mg·kg^-1,以丹参酮ⅡA计),每组10只。采用线栓法制备大鼠局灶性脑缺血再灌注损伤模型,缺血2 h、再灌注22 h后检测大鼠神经功能评分,TTC染色检测大鼠脑梗死面积百分比,HE染色检测大鼠脑组织病理形态学,检测大鼠脑组织匀浆中超氧化物歧化酶(SOD)活性、丙二醛(MDA)及一氧化氮(NO)水平。结果:与假手术组比较,模型组大鼠神经功能评分升高(P<0.05),脑梗死面积百分比增大(P<0.05),脑组织中SOD活性明显降低(P<0.01),MDA和NO水平明显升高(P<0.01)。与模型组比较,中、高剂量DSLZ组大鼠神经功能评分降低,脑梗死面积百分比明显缩小,脑组织中 SOD活性明显升高, MDA及NO水平明显降低(P<0.05或 P<0.01)。HE染色,模型组大鼠神经细胞胞膜不清,细胞出现肿胀、变形、坏死,细胞间质水肿,细胞间隙增大;高剂量DSLZ组大鼠神经细胞核仁清晰,间质轻微水肿。结论:DSLZ可减轻脑缺血大鼠神经细胞损伤,其机制可能与减轻自由基损伤、抑制NO神经毒性有关联。

关键词: 丹参磷脂, 脑缺血灌注, 氧化应激, 超氧化物歧化酶, 丙二醛

Abstract:

Objective To investigate the protective effect of Danshenlinzhi(DSLZ) on the cerebral ischemia reperfusion injury in the rats, and to explore its mechanism.Methods 60 Wistar rats were randomly divided into sham group,model group,TanⅡA group, and low,middle,high, doses (5.0,10.0,20.0 mg·kg^-1) of DSLZ groups(n=10). The thread embolism was performed to establish the rat models of focal cerebral ischemia reperfusion injury. The neurologic scores of the rats after 2 h ischemia and 22 h reperfusion were measured and the percentages of the cerebral infarction area of the rats were detected by TTC staining method; the pathomorphology of the brain tissue of the rats was detected by HE staining. The activities of superoxide dismutase (SOD) and levels of malondialdehyde(MDA),nitric oxide (NO) in the brain tissue of the rats were detected.Results Compared with sham group,the neurologic score of the rats in model group was increased(P<0.05),the percentages of the cerebral infarction area was increased(P<0.05),the activity of SOD was decreased(P<0.01) and the levels of MDA and NO were increased (P<0.01). Compared with model group,the neurologic scores of the rats in middle and high doses of DSLZ groups were decreased and the percentages of the cerebral infarction area were decreased,the activities of SOD were increased and the levels of MDA and NO were decreased(P<0.05 or P<0.01).The HE staining results showed the membrane of neurocyte was unclear,and the cells became swelling,deformation or necrosis,severe edema in interstitium and the cell space was enlarged in model group;the nucleolus could be found clearly and there was slight edema in interstitium of the cells of the rats in high dose of DSLZ group.Conclusion DSLZ has a protective effect on the cerebral ischemia reperfusion injury of the rats,and its mechanism may be related to the anti-radical damage and lessening the neurotoxicity of NO.

Key words: Danshenlinzhi, cerebral ischemia reperfusion injury, oxidative stress, superoxide dismutase, malondialdehyde

中图分类号:

R541.4

曲圆, 付丹阳, 纪影实. 丹参磷脂浸膏对大鼠脑缺血再灌注损伤的保护作用及其机制[J]. 吉林大学学报(医学版), 2015, 41(02): 291-294.

QU Yuan, FU Danyang, JI Yingshi. Protective effect of Danshenlinzhi on cerebral ischemia reperfusion injury in rats and its mechanism[J]. Journal of Jilin University Medicine Edition, 2015, 41(02): 291-294.

参考文献

[1] 张家堂,郎森阳,匡培根,等.丹参对大鼠局灶性脑缺血后神经元内细胞周期蛋白D1表达的影响[J]. 中华老年心脑血管病杂志,2001,3(4):272-275.

[2] 罗建,王晔,程薇.磷脂酰胆碱对小鼠记忆障碍模型的影响[J].现代预防医学,2011,38(3):446-448.

[3] Wu LF,Liu YY,Fang SH,et al. Protective effect of recombinant human glutaredoxin-1 against the cerebral ischemia reperfusion injury in mice[J].Chin Pharmacol Bull,2007,23(5):641-643.

[4] Tu Q,Wang R,Qing B.Protective and antioxidant effect of Dan-shen polysaccharides on cerebral ischemia/reperfusion injury in rats[J].Int J Biol Macromol,2013,60:268-271.

[5] Tan H,Huang XP,Deng CQ. Effects of astragalosides and Panax notogiseng saponins combination on oxidative stress of cerebral ischemic reperfusion injury in mice[J].J Chin Integr Med,2010,8(5):448-452.

[6] 陈正跃,许建文,张光军.水飞蓟宾-磷脂酰胆碱复合物对大鼠脑缺血再灌注损伤的保护作用[J].中国临床康复,2004,8(22):4631-4633.

[7] Pan C,Lou L,Huo Y,et al.Salvianolic acid B and tanshinone ⅡA attenuate myocardial ischemia injury in mice by NO production through multiple pathways [J].Ther Adv Cardiovasc Dis,2011,5(2):99-111.

[8] Wang TF,Lei Z,Li YX,et al.Oxysophoridine protects against focal cerebral ischemic injury by inhibiting oxidative stress and apoptosis in mice [J].Neuroche Res,2013,38(11):2408-2417.

[9] Dang S,Liu X,Fu P,et al.Neuroprotection by local intra-arterial infusion of erythropoietin after focal cerebral ischemia in rats[J].Neurol Res,2011,33(5):520-528.

[10] Xu W,Yang J,Wu LM. Cardioprotective effects of tanshinone IIA on myocardial ischemia injury in rats [J].Pharmazie,2009,64(5):332-337.

[11] 董文鹏,章翔,高大宽,等. 白藜芦醇对小鼠局灶性缺血/再灌注后急性期的影响[J].中国药理学通报,2008,24(8):1032-1035.

[12] 李红,张爽,纪影实,等.山楂叶总黄酮对大鼠局灶性脑缺血再灌注损伤的保护作用[J].中草药,2010,41(5):794-796.

[13] 邓永超,刘煜敏.银杏叶提取物后处理对大鼠局灶性脑缺血再灌注损伤保护作用的研究[J]. 卒中与神经疾病,2014,21(4):219-221.

[14] 刘凤琴,牛小媛,牛文华.大鼠脑缺血再灌注后脑组织水通道蛋白的表达及丹参川芎嗪干预作用的研究[J].中华老年心脑血管病杂志,2012,12 (3):123-126.

[15] Wen QW,Liang L,Hui CS,et al.Tanshinone ⅡA inhibits metastasis after palliative resection of hepatocellular carcinoma and prolongs survival in part via vascular normalization[J].J Hematol Oncol,2012,5:69.doi:10.1186/1756-8722-5-69.

[16] 尚涛,杜玉茗,姚庆青,等.吡格列酮联合丹参对糖尿病脂肪肝患者糖、脂代谢及肝损伤的影响[J].吉林中医药,2013,33(4):374-375. *吉林大学临床医学院2011级临床医学专业

丹参磷脂浸膏对大鼠脑缺血再灌注损伤的保护作用及其机制

Protective effect of Danshenlinzhi on cerebral ischemia reperfusion injury in rats and its mechanism

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	陈雪, 沈楠, 安英, 徐博, 赵丽晶. 丹酚酸B对氧化应激损伤乳鼠心肌细胞的保护作用及其机制[J]. 吉林大学学报(医学版), 2018, 44(05): 974-978.
[2]	冉琴, 张雷, 张沄, 邱玉环, 袁谢芳, 王孝芸, 唐红梅, 王星, 李国平. 白藜芦醇对中性粒细胞性哮喘小鼠肺组织炎症的抑制作用及其机制[J]. 吉林大学学报(医学版), 2018, 44(05): 897-902.
[3]	黄凯文, 李阿荣, 邓志军, 刘若轩, 李常青, 钟世顺, 郭洁文, 张洁, 陈滨. 荔枝核皂苷对糖调节受损大鼠胰腺组织氧化应激的改善作用及其机制[J]. 吉林大学学报(医学版), 2018, 44(04): 746-752.
[4]	苑荣爽, 李贺, 孙靖辉, 庄文越, 陈建光, 王春梅. 北五味子多糖对高脂诱导非酒精性脂肪性肝病大鼠的降血脂作用及其抗氧化活性[J]. 吉林大学学报(医学版), 2017, 43(06): 1103-1108.
[5]	石立强, 陈丽娜, 李洪宇, 谢立亚, 糜心雅, 苑广信, 孙晶波, 王曼力, 徐广宇, 韩笑, 赵南晰, 盛瑜, 杜培革, 安丽萍. 高活性小牛血去蛋白提取物对小鼠酒精性肝损伤的保护作用[J]. 吉林大学学报(医学版), 2016, 42(04): 742-745.
[6]	张博男, 张静怡, 储金秀, 王树华, 韩淑英. 荞麦花叶芦丁对高糖刺激血管损伤的保护作用[J]. 吉林大学学报(医学版), 2016, 42(01): 40-47.
[7]	黄晓东, 沈楠, 路倩, 刘微, 常影, 范红艳, 王艳春. 黄芪甲苷对糖尿病肾脏病变大鼠氧自由基代谢和转化生长因子β1mRNA表达的影响[J]. 吉林大学学报(医学版), 2016, 42(01): 48-53.
[8]	米亚静, 刘洁, 王世伟, 王天伟, 景晓红. DNA 双加氧酶TET2在老年痴呆动物模型脑组织中的表达及其对氧化应激中神经元的保护作用[J]. 吉林大学学报(医学版), 2015, 41(04): 727-731.
[9]	刘黎星, 闵羡慧, 张敏, 胡建章. 子痫前期患者血清中ABCA1的表达及其对内皮细胞损伤的影响[J]. 吉林大学学报(医学版), 2015, 41(03): 448-453.
[10]	于洋, 徐冶, 刘师兵, 衣丹, 李松岩, 王晓军. 过氧化氢通过内质网应激信号通路对Hela细胞自噬和凋亡的诱导作用[J]. 吉林大学学报(医学版), 2015, 41(03): 437-441.
[11]	姜恩平, 于春荣, 李贺, 于春艳, 陈建光. 北五味子总木脂素对PC12细胞氧化应激损伤的保护作用及其抑制NF-κB/iNOS/NO信号通路的机制[J]. 吉林大学学报(医学版), 2015, 41(03): 532-536.
[12]	吴冠宇, 王硕, 刘艳, 赵淑华. 邻苯二甲酸二丁酯对雄性小鼠生殖系统的脂质过氧化作用[J]. 吉林大学学报(医学版), 2015, 41(03): 553-557.
[13]	徐姗姗, 朴美花, 王艳姝, 刘楠, 裴爱月, 冯春生. 异氟醚对Aβ25-35诱导大鼠PC12细胞氧化应激损伤的影响及海藻糖的保护作用[J]. 吉林大学学报(医学版), 2015, 41(02): 207-212.
[14]	勾向博, 白静, 郭静, 韩淑英. 复方苦荞麦制剂对大鼠糖尿病心肌损伤的保护作用[J]. 吉林大学学报(医学版), 2015, 41(01): 83-87.
[15]	王司仪,原铭贞,刘笑玎,刘相良,高歌,梁豪君,李波,董春玲. 细胞因子与肺纤维化相关性的研究进展[J]. 吉林大学学报(医学版), 2014, 40(06): 1325-1329.