吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (04): 737-741.doi: 10.13481/j.1671-587x.20150413

• 基础研究 • 上一篇    下一篇

氧化樟脑注射液对大鼠心肌纤维化的改善作用

安英1, 杨倩2, 黄健禄2, 江凯2, 曾庆容3, 宋文欣4, 王艳春5   

  1. 1. 吉林医药学院基础医学院机能教研室, 吉林 吉林 132013;
    2. 吉林医药学院口腔影像系, 吉林 吉林 132013;
    3. 吉林医药学院临床医学院, 吉林 吉林 132013;
    4. 吉林医药学院检验学院, 吉林 吉林 132013;
    5. 吉林医药学院基础医学院药理学教研室, 吉林 吉林 132013
  • 收稿日期:2014-10-12 发布日期:2015-08-01
  • 通讯作者: 王艳春,教授,硕士研究生导师(Tel:0432-64560305,E-mail:wangyanchun1972@163.com) E-mail:wangyanchun1972@163.com
  • 作者简介:安英(1979-),女,吉林省延吉市人,讲师,理学硕士,主要从事心血管药理学方面的研究。
  • 基金资助:

    国家自然科学基金资助课题(30873066);吉林省教育厅科学技术研究项目资助课题(2009311);吉林省教育厅大学生创新创业训练计划项目资助课题(2013)

Improvement effect of Vitacamphorae Injection on myocardial fibrosis in rats

AN Ying1, YANG Qian2, HUANG Jianlu2, JIANG Kai2, ZENG Qingrong3, SONG Wenxin4, WANG Yanchun5   

  1. 1. Medical Functional Laboratory, School of Basic Medical Science, Jilin Medical College, Jilin 132013, China;
    2. Department of Oral Imaging, Jilin Medical College, Jilin 132013, China;
    3. School of Clinical Medicine, Jilin Medical College, Jilin 132013, China;
    4. School of Laboratory, Jilin Medical College, Jilin 132013, China;
    5. Department of Pharmacology, School of Basic Medical Science, Jilin Medical College, Jilin 132013, China
  • Received:2014-10-12 Published:2015-08-01

摘要:

目的: 探讨氧化樟脑注射液对异丙肾上腺素(Iso)所致大鼠心肌纤维化的作用,阐明其抗心肌纤维化的作用机制,为其临床应用奠定基础。方法: 60只SD大鼠随机分为对照组、Iso模型组、维生素C组和不同剂量氧化樟脑注射液组;采用2 mg·kg-1·d-1Iso皮下注射制备大鼠心肌纤维化模型,连续14d,不同剂量氧化樟脑注射液组和维生素C组大鼠同时腹腔注射氧化樟脑注射液 (0.8、1.6和3.2 mg·kg-1·d-1)和维生素C(80 mg·kg-1·d-1),连续28 d。实验结束后采用BL-420 E+生物机能实验系统检测各组大鼠心电图和心功能,测定心脏质量指数(HMI)和左心室质量指数(LVMI);紫外分光法检测心肌组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)和诱导型一氧化氮合酶(iNOS)活性;酶标仪检测心肌组织中乳酸脱氢酶(LDH)活性和一氧化氮(NO)含量;ELISA法检测心肌组织中Ⅰ型胶原(ColⅠ)和Ⅲ型胶原(Col Ⅲ)含量;HE染色观察心肌组织病理学。结果: 与对照组比较,Iso模型组大鼠心率增快、左室收缩压(LVSP)降低、左室内压最大上升速率(LVdp/dtmax)降低(P<0.05或P<0.01);HMI和LVMI升高,但差异无统计学意义(P>0.05);左心室心肌组织中MDA含量和LDH活性增高, NO含量降低,SOD和iNOS活性降低,ColⅠ和Col Ⅲ含量增高(P<0.05或P<0.01)。与Iso模型组比较,不同剂量氧化樟脑注射液组大鼠HMI和LVMI降低,且呈剂量依赖性,但差异无统计学意义(P>0.05);不同剂量氧化樟脑注射液组大鼠心肌组织匀浆中LDH活性、MDA含量降低,NO含量增加(P<0.05或P<0.01),ColⅠ和Col Ⅲ含量降低(P<0.05或P<0.01),iNOS和SOD活性升高(P<0.05或P<0.01)。结论: 氧化樟脑注射液可抑制Iso所致的大鼠心肌纤维化,其机制可能与抗氧化、提高NO水平进而抑制胶原的合成有关。

关键词: 心肌纤维化, 氧化樟脑注射液, 异丙肾上腺素

Abstract:

Objective To explore the effect of Vitacamphorae Injection on the myocardial fibrosis induced by isoproterenol(Iso) in the rats,and to clarify its mechanism of anti-myocardial fibrosis,and to provide the basis for its clinical application. Methods 60 SD rats were randomly divided intocontrol group,Iso model group, vitamin C group and different doses of Vitacamphorae Injection groups. The models of myocardial fibrosis in the rats were set up by using subcutaneous injection of 2 mg·kg-1·d-1 Iso,for 14 consecutive days,while the rats in different doses of Vitacamphorae Injection groups and vitamin C group were subcutaneouly injected with 0.8,1.6,3.2 mg·kg-1·d-1 vitacamphorae and 80 mg·kg-1·d-1 vitamin C,for 28 consecutive days. BL-420 E+ biological function experimental system was used to detect the rat ECG and heart function after experiment. Thenthe heart mass index (HMI) and left ventricular mass index (LVMI) were measured. The contents of malondialdehyde (MDA),the activities of superoxide dismutase (SOD) and inducible nitric oxide synthase enzyme (iNOS)in the myocardium tissue were measured by UV detection.The activities of the lactate dehydrogenase (LDH) and the contents of nitric oxide (NO) were examined by enzyme labelling instrument.The contents of type Ⅰ (ColⅠ) collagen and type Ⅲ collagen (Col Ⅲ) in the myocardium tissue of the rats were detected by ELISA.The pathological changes of myocardium tissue were observed by HE staining. Results Compared with control group,the heart rate of the rats in Iso model group was increased,the left ventricular systolic pressure (LVSP) was decreased,and the maximum rising rate of left ventricular pressure (LVdp/dtmax) was decreased (P<0.05 or P<0.01); the HMI and LVMI were increased,but there were no significant differences(P>0.05).In left ventricular myocardium tissue,the contents of MDA,Col Ⅰ and Col Ⅲ were increased,while the NO content was decreased(P<0.05 or P<0.01),and the activities of SOD and iNOS were decreased,but the LDH activity was increased(P<0.05 or P<0.01).Compared with Iso model group,the HMI and LVMI of the rats in different doses of Vitacamphorae Injection groups were decreased in a dose-dependent manner;and the contents of MDA,the LDH activities,the Col Ⅰ and Col Ⅲ contents were decreased(P<0.05 or P< 0.01),while the NO contents and the activities of iNOS,SOD were increased(P<0.05 or P<0.01). Conclusion Vitacamphorae Injection could inhibit the myocardial fibrosis induced by Iso in the rats,and its mechanism may be related to antioxidant and increasing the level of NO to inhibit the synthesis of collegen.

Key words: myocardial fibrosis, Vitacamphorae Injection, isoproterenol

中图分类号: 

  • R542.23