吉林大学学报(医学版) ›› 2013, Vol. 39 ›› Issue (3): 503-506.doi: 10.7694/jldxyxb20130316

• 基础研究 • 上一篇    下一篇

银杏叶提取物对STZ和ISO协同诱发大鼠糖尿病心肌病的治疗作用

刘 伟1,2，陈 韬3，韩洪波2，朱 昆2，马铭泽2，王丹萍2，高海成4，金明玉1   

  1. 1．延边大学中医学院中医基础教研室，吉林 延吉 133000；2.解放军第208医院肾脏内分泌科，吉林 长春130062；3.空军航空大学内科，吉林 长春130022； 4.吉林大学药学院临床药学教研室，吉林 长春130021
  • 收稿日期:2013-01-25 出版日期:2013-05-28 发布日期:2013-07-01
  • 通讯作者: 金明玉（Tel：0431-85619702，E-mail：kimmy@ybu.edu.cn） E-mail:kimmy@ybu.edu.cn
  • 作者简介:张洪长(1961-),男,吉林省长春市人，副研究员，医学博士，主要从事分子 生物化学与药理学方面的研究。
  • 基金资助:

     吉林省科技厅科技发展计划项目资助课题（200905102）

Therapeutic effect of gink gobioba extract  on diabetic cardiomyopathy induced by SZT and  ISO  in rats

LIU Wei 1,2,CHEN Tao3,HAN Hong-bo2,ZHU Kun2,MA Ming-ze2,WANGDan-ping2,GAO Hai-cheng4,JIN Ming-yu1   

  1. 1. Department of  Basic Traditional Chinese Medicine ，College of Traditonal Chinese  Medicine,Yanbian University,Yanji 133000,China;2. Department of Endocrinology and Kidney, No. 208  Hospital of PLA,Changchun 130062,China;3.Deparment of  Internal Medicine,Aviation University of Air Force，Changchun 130022,China;4.Deparment of Clinical Pharmacy，School of Pharmacy,Jilin University,Changchun 130021,China
  • Received:2013-01-25 Online:2013-05-28 Published:2013-07-01

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摘要： 目的:  探讨银杏叶提取物(GBE) 对链脲佐菌素（STZ）和盐酸异丙肾上腺素(ISO)协同诱发大鼠糖尿病心肌病(DCM)的治疗作用。方法： 20只雄性Wistar大鼠随机分为正常对照组、模型组、阳性对照组和治疗组，每组5只。正常对照组大鼠不予处理，其他3组大鼠给予STZ和ISO造模，模型组大鼠给予生理盐水灌胃，阳性对照组和治疗组大鼠造膜后分别灌胃给予盐酸贝那普利和GBE，观察各组大鼠体质量、血糖水平、血清天冬氨酸氨基转移酶（AST）、乳酸脱氢酶（LDH）、转化生长因子β1（TGF-β1）蛋白表达水平和心肌组织形态学的变化。结果：给药1和2周时，模型组大鼠体质量、血糖水平较正常对照组降低(P<0.05），阳性对照组和治疗组大鼠体质量、血糖水平大鼠较模型组降低(P<0.05）。模型组大鼠AST、LDH和 TGF-β1水平较正常对照组降低（P<0.05），阳性对照组和治疗组大鼠AST、LDH和 TGF-β1水平较模型组升高（P<0.05）。正常对照组大鼠心肌无细胞肿胀；模型组大鼠心肌细胞出现界限清晰、多发散在坏死灶，坏死灶内有旺盛的成纤维细胞增生，并可见一定量的胶原纤维；治疗组大鼠心肌细胞坏死病灶面积减小，纤维化程度减轻。结论：GBE可以缓解STZ和ISO协同诱导的大鼠心肌损害。

关键词: 银杏叶提取物, 链脲佐菌素, 盐酸异丙肾上腺素, 转化生长因子-&beta, 1

Abstract: Abstract:Objective To investigate the therapeutic  effect of gink gobioba extract (GBE) on diabetic cardiomyopathy (DCM) induced by streptozotocin (SZT) and isoprenaline (ISO) in rats. Methods 20 male Wistar rats were randomly divided into normal control group(n=5),model group(n=5),positive control group(n=5),and treatment group (n=5).The rats in normal control group were given nothing and the other rats were given STZ and ISO to construct DCM rat models.The rats in model group were given saline and the rats in positive control group and treatment groupwere given benazepril and GBE,respectively.The body weight,blood sugar level,serum AST and LDH levels,expression level of TGF-β1 protein,and morphology of myocardium of rats  in various groups were observed. Results 1 and 2  weeks after treatment,the body weight and blood sugar level of the  rats  in model group were lower than those in  normal control group（P<0.05）. The body weights and blood sugar levels of the rats in positive control and treatment group were lower than those in  normal control group（P<0.05）. The levels of AST,LDH,and TGF-β1 in model group were higher than those in   normal group(P<0.05）;and the levels of AST,LDH,and TGF-β1 in positive control and treatment group were higher than those in model group(P<0.05).The myocardial  cells of the  rats in normal control group had no swelling.The cells in model group showed clear boundaries and more scattered necrosis,and there were lots of fibroblasts  and some collagen fibers in focal necrosis.The myocardial cells of the  rats intreatment group had smaller focal necrosis and lower fibrosis.Conclusion GBE can alleviate myocardial injury induced by STZ plus ISO of rats.

Key words: ginkgo biloba extract, streptozotocin, isoproterenol, transforming growth factor-&beta

中图分类号: 

  • R587
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