吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (06): 1163-1170.doi: 10.13481/j.1671-587x.20150613

• 基础研究 • 上一篇    下一篇

红景天和党参合剂对小鼠免疫功能的影响

马天宇1, 姜国哲2, 韩春姬2, 刘丽萍3, 俞笃文4,5   

  1. 1. 延边大学医学院预防医学教研室, 吉林 延吉 133002;
    2. 延边大学医学院免疫学与病原生物学教研室, 吉林 延吉 133002;
    3. 延边大学医学院生理学教研室, 吉林 延吉 133002;
    4. 嘉南药理大学药学院药学系, 台湾 台南 71710;
    5. 台湾丰山生技医药品股份公司, 台湾 台北 10597
  • 收稿日期:2015-06-17 发布日期:2016-01-11
  • 通讯作者: 韩春姬,教授,博士研究生导师(Tel:0433-2435076,E-mail:cjhan@ybu.edu.cn);俞笃文,助理教授,硕士研究生导师(Tel:886-2-27682713,E-mail:vincent@fungsun.com) E-mail:cjhan@ybu.edu.cn;vincent@fungsun.com
  • 作者简介:马天宇(1990-),女,吉林省延吉市人,在读医学硕士,主要从事分子毒理学及保健食品研发方面的研究。
  • 基金资助:

    科技部国际合作专项基金资助课题(2011DFA33470);吉林省科技厅科技发展计划项目资助课题(200905208)

Influence ofRhodiolasachalinensis and Codonopsispilosula mixtureinimmune function of mice

MA Tianyu1, JIANG Guozhe2, HAN Chunji2, LIU Liping3, YU Duwen4,5   

  1. 1. Department of Preventive Medicine, College of Medical Sciences, Yanbian University, Yanji 133002, China;
    2. Department of Immunology and Pathogenic Biology, College of Medical Sciences, Yanbian University, Yanji 133002, China;
    3. Department of Physiology, College of Medical Sciences, Yanbian University, Yanji 133002, China;
    4. Department of Pharmacy, College of Pharmacy, Chia Nan University, Tainan 71710, Taiwan;
    5. Fun Sun Medical and Pharmaceutical Technology Co., Ltd., Taipei 10597, Taiwan
  • Received:2015-06-17 Published:2016-01-11

摘要:

目的:应用血清药理学方法探讨红景天提取物(RSE)与党参提取物(CPE)合剂对免疫功能的影响,分析提高免疫功能的最佳配伍剂量。方法:采用3×3析因设计,制备9种不同浓度的CPE和RSE混合含药血清,在体外测定T、B 淋巴细胞增殖活性和 NK 细胞活性,确定CPE和RSE的最佳组合剂量。用该组合剂量缩小2.5、5.0及10.0倍作为RSE+CPE合剂高、中和低剂量组,进行小鼠体内实验,检测T、B 淋巴细胞的增殖能力和NK 细胞的杀伤能力。结果:血清药理学实验,与空白组比较,RSE 200 mg·kg-1+CPE 790 mg·kg-1剂量组由ConA、LPS诱导的T、B淋巴细胞增殖能力及NK细胞活性明显升高(P<0.05)。小鼠体内实验,与环磷酰胺组比较,RSE+CPE合剂中(RSE40 mg·kg-1+CPE 158 mg·kg-1)和高剂量(RSE 80 mg·kg-1 + CPE 316 mg·kg-1)组脾脏指数(SI)明显升高(P<0.05);与RSE+合剂低剂量(RSE20 mg·kg-1+CPE 79 mg·kg-1)组比较,合剂中剂量组白细胞数明显升高(P<0.05);与环磷酰胺组比较,CPE合剂高、中和低3个剂量组及阳性药物CVT-200(富含西洋参多糖的免疫增强剂)组ConA、LPS诱导的T、B淋巴细胞增殖能力及NK细胞活性均显著升高(P<0.05);与CVT-200组、合剂高剂量和低剂量组比较,合剂中剂量组T、B淋巴细胞增殖能力及NK细胞活性均显著升高(P<0.05)。结论:RSE和CPE合剂有增强小鼠免疫功能的作用,RES 200 mg·kg-1与CPE790mg·kg-1组合的合剂为最佳配伍剂量。

关键词: 红景天, 党参, 免疫功能

Abstract:

Objective Using serum pharmacology method to assess the optimal dose of Rhodiolasachalinensis extract(RSE)and Codonopsispilosula extract(CPE)combination as well as the effects of the combination on the immune function of mice. Methods Using the 3×3 facterial design,nine medicated serum of CPE and RSE mixture were made to assess the optimum combinational dose of CPE and RSE by detecting the T,B lymphocyte proliferation abilities and NK cell activity in vitro.Using the optimum combinational dose and reducing 2.5,5 and 10 times as high,middle and low doses of RSE+CPE groups,and the T,B lymphocyte proliferation abilities and the activity of NK cells in the mice were detected in vivo. Results The result of serum pharmacology indicates that compared with control group,the proliferation abilities of T,B lymphocytes and the activity of NK cells inducing by ConA and LPS in 200 mg·kg-1 RSE +790 mg·kg-1 CPE group were increased (P<0.05).The result of in vivo experiment indicated that compared with cyclophosphamide group,the spleen indexes(SI) in middle and high doses of RSE+CPE groups were significantly increased (P<0.05); compared with low dose of RSE+CPE group,the WBC number in middle dose of RSE+CPEgroup was significantly increased (P<0.05).Compared with cyclophosphamide group,the T and B cell proliferation abilities induced with ConA and LPS and killing activities of NK cells in low,middle and high doses of RSE+CPE groups and positive drug CVT-200 group were significantly increased (P<0.05).Compared with CVT-200 group,low and high doses of RSE+CPE groups,the T and B cell proliferation abilities induced with ConA and LPS and the activity of NK cells in middle dose of RSE+CPE group were significantly increased(P<0.05). Conclusion RSE and CPEmixture can enhance the immune function of mice; RES 200 mg·kg-1 and CPE 790 mg·kg-1 are the optimal doses.

Key words: Rhodiolasachalinensis, Codonopsispilosula, serum pharmacology, immune function

中图分类号: 

  • R285