吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (06): 1158-1162.doi: 10.13481/j.1671-587x.20150612

• 基础研究 • 上一篇    下一篇

黄芩苷对人结肠癌SW480细胞凋亡的诱导作用及其机制

赖明广1, 青海涛2, 王立生3, 刘圣活1   

  1. 1. 广东省深圳市龙岗区中医院腔镜中心, 广东 深圳 518100;
    2. 南方医科大学南方医院消化内科, 广东 广州 510515;
    3. 暨南大学第二临床医学院消化内科, 广东 深圳 518020
  • 收稿日期:2015-05-06 发布日期:2016-01-11
  • 通讯作者: 赖明广,主治医师(Tel:0755-89911830,E-mail:lmg198002@163.com) E-mail:lmg198002@163.com
  • 作者简介:赖明广(1980-),男,广东省深圳市人,主治医师,医学博士,主要从事大肠癌的基础与临床方面的研究。
  • 基金资助:

    国家自然科学基金资助课题(81201662)

Induction effect of baicalin on apoptosis of human colon cancer SW480 cells and its mechanism

LAI Mingguang1, QING Haitao2, WANG Lisheng3, LIU Shenghuo1   

  1. 1. Laparoscope Center, Shenzhen Longgang Hospital of TCM, Guangdong Province, Shenzhen 518100, China;
    2. Department of Gastroenterology, Nanfang Hospital, Nanfang Medical University, Guangzhou 510515, China;
    3. Department of Gastroenterology, Jinan University of Medical Sciences, Shenzhen Municipal People's Hospital, Shenzhen 518020, China
  • Received:2015-05-06 Published:2016-01-11

摘要:

目的:探讨黄芩苷对人结肠癌SW480细胞凋亡的影响,并阐明其作用机制。 方法:SW480细胞分为空白对照组,25、50和100 μmol·L-1黄芩苷组,采用CCK-8法检测SW480细胞增殖活性,Annexin Ⅴ-FITC和DAPI染色观察SW480细胞凋亡的形态学表现,Western blotting法检测凋亡蛋白Bcl-2、caspase 3和caspase 9表达水平。 结果:与空白对照组比较,50和100 μmol·L-1黄芩苷组SW480细胞增殖活性在24、48和72 h均明显降低( P<0.01),25 μmol·L-1黄芩苷组SW480细胞增殖活性在48和72 h明显降低( P<0.01)。50 μmol·L-1黄芩苷作用48 h时SW480细胞处于早期或晚期凋亡状态,细胞核呈浓缩和破碎状态。与空白对照组比较,25、50和100 μmol·L-1黄芩苷组caspase 3和caspase 9蛋白表达水平明显升高 (P<0.05或P<0.01),Bcl 2蛋白表达水平明显降低 (P<0.05或P<0.01)。结论:黄芩苷可以诱导SW480细胞凋亡,其机制可能与线粒体途经有关。

关键词: 黄芩苷, SW480细胞, 细胞凋亡, Bcl 2, caspase 3, caspase 9

Abstract:

Objective To investigate the influence of baicalin in human colon cancer SW480 cells,and to clarify its mechanism. Methods The SW480 cells were cultured and divided into blank control and 25,50 and 100 μmol·L-1 baicalin groups.The proliferation activity was detected with CCK-8 assay.The morphological changes of SW480 cells were detected by Annexin Ⅴ-FITC and DAPI coloration.The protein expression levels of Bcl-2,caspase 3 and caspase 9 were detected by Western blotting method. Results The CCK-8 assay results showed that the proliferation activities of SW480 cells in 25,50 and 100 μmol·L-1 baicalin groups were decreased significantly compared with blank control group at the time points of 24 h,48 h and 72 h (P<0.01),the proliferation activities of SW480 cells in 25 μmol·L-1 baicalin groups were decreased significantly compared with blank control group at the time points of 48 and 72 h (P<0.01).Cell shrinkage and nucleus fragmentation were observed in the SW480 cells after treated with 50 μmol·L-1 baicalin for 48 h.The Western blotting assay results showed that compared with blank control group,the protein expression levels of caspase 3 and caspase 9 in 25,50 and 100 μmol·L-1 baicalin groups were increased significantly (P<0.05 or P<0.01),and the protein expression levels of Bcl 2 in 25,50 and 100 μmol·L-1 baicalin groups were decreased significantly (P<0.05 or P<0.01). Conclusion Baicalin can induce the apoptosis in SW480 cells,and the effect might be involved with the mitochondrial apoptotic pathway.

Key words: baicalin, SW480 cells, apoptosis, Bcl-2, caspase 3, caspase 9

中图分类号: 

  • R363.2