宫颈鳞状细胞癌组织中miRNA差异性表达及其靶基因作为诊断标志物的意义

doi:10.13481/j.1671-587x.20160117

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (01): 85-88.doi: 10.13481/j.1671-587x.20160117

宫颈鳞状细胞癌组织中miRNA差异性表达及其靶基因作为诊断标志物的意义

于飞飞, 田雨溪, 刘晓冬, 马淑梅

吉林大学公共卫生学院卫生部放射生物学重点实验室, 吉林长春 130021

收稿日期:2015-06-21 发布日期:2016-01-26
通讯作者: 马淑梅,教授,硕士研究生导师(Tel:0431-85619443,E-mail:shmm2001@126.com) E-mail:shmm2001@126.com
作者简介:于飞飞(1990-),女,山东省德州市人,在读医学硕士,主要从事放射生物学方面的研究。
基金资助:
国家自然科学基金资助课题(31370837);吉林省卫生计生委基金资助课题(20142036);吉林省科技厅科研基金资助课题(201205010)

Differential expression of miRNA in cervical squamous cell carcinoma tissue and significance of its target genes as diagnostic markers

YU Feifei, TIAN Yuxi, LIU Xiaodong, MA Shumei

Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China

Received:2015-06-21 Published:2016-01-26

摘要/Abstract

摘要：

目的: 采用生物信息学方法研究宫颈鳞状细胞癌(CESC)组织中差异性表达的miRNA并预测其靶基因,以期找到影响CESC发生发展及可用于肿瘤标志物的miRNA。方法: 癌症基因组图谱 (TCGA)数据库中下载3例CESC组织及3例配对正常组织,用统计学方法对差异性表达的基因及miRNA进行筛选,通过靶基因预测网站对差异性表达的miRNA进行靶基因预测,运用KEGG数据库分析靶基因参与的肿瘤相关信号通路。结果: 与同源正常组织比较,CESC组织有18个miRNA和1180个基因有差异性表达(P<0.05),其中有15个miRNA和411个基因上调,3个miRNA和770个基因下调,采用靶基因预测网站有7个miRNA与预测到的靶基因呈负向调控关系,6个上调miRNA的靶基因下调,1个下调miRNA的靶基因上调,靶基因集中在Wnt、MAPK、P53和cAMP等肿瘤相关信号通路。结论: 差异性表达的miRNA包括hsa-miR-27a、hsa-miR-148b、hsa-miR-185、hsa-miR-200a、hsa-miR-200b、hsa-miR-221和hsa-mir-133b,差异性表达的miRNA及其靶基因在CESC的发生发展过程中起重要作用,有可能成为诊断CESC的肿瘤标志物。

关键词: 宫颈鳞状细胞癌, 微小RNA, 肿瘤标志物

Abstract:

Objective: To study the differential expression of miRNA in cervical squamous cell carcinoma(CESC)tissue and predict its target genes using bioinformatics methods,and to find the miRNA as tumor markers in the pathological process of CESC.Methods: The CESC samples and their matching normal CESC tissue in TCGA database were download,and the differential expression of genes and miRNAs were screened with statistical method,and the target gene prediction was performed for the differently expressed miRNA with target gene prediction websites.KEGG database was used to analyze the target genes involved in tumor related signaling pathway.Results: Compared with their matching normal CESC tissue,21 miRNA and 1 180 genes were found to be differentially expressed(P<0.05),18 miRNA and 411 genes were up-regulated,and 3 miRNA and 770 genes were down-regulated.7 miRNAs had negative correlationship with the predicted target genes;6 up-regulated miRNA were accompanied by their target genes down-regulation,and down-regulated 1 miRNA were accompanied by their target genes up-regulation;their target genes were involved in Wnt,MAPK,P53 and cAMP tumor related signaling pathways.Conclusion: The differentially expressed miRNAs including hsa-miR-27a,hsa-miR-148b,hsa-miR-185,hsa-miR-200a,hsa-miR-200b,hsa-miR-221 and hsa-mir-133b and their target genes play important roles in the development of CESC,and they are possible to become the tumor markers in the diagnosis of CESC.

Key words: cervical squamous cell carcinoma, microRNA, tumor marker

中图分类号:

R737.33

于飞飞, 田雨溪, 刘晓冬, 马淑梅. 宫颈鳞状细胞癌组织中miRNA差异性表达及其靶基因作为诊断标志物的意义[J]. 吉林大学学报(医学版), 2016, 42(01): 85-88.

YU Feifei, TIAN Yuxi, LIU Xiaodong, MA Shumei. Differential expression of miRNA in cervical squamous cell carcinoma tissue and significance of its target genes as diagnostic markers[J]. Journal of Jilin University Medicine Edition, 2016, 42(01): 85-88.

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宫颈鳞状细胞癌组织中miRNA差异性表达及其靶基因作为诊断标志物的意义

Differential expression of miRNA in cervical squamous cell carcinoma tissue and significance of its target genes as diagnostic markers

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