胶质瘤患者T淋巴细胞表面CTLA-4的表达及其临床意义

doi:10.13481/j.1671-587x.20160223

摘要/Abstract

摘要：

目的: 探讨胶质瘤患者外周血中CD4⁺和CD8⁺T细胞表面CTLA-4表达与胶质瘤WHO分级及手术的关系,阐明CTLA-4的临床意义。方法: 选择本院胶质瘤患者60例作为胶质瘤组,健康志愿者46名作为健康对照组。流式细胞术检测胶质瘤组和健康对照组研究对象外周血中CD4⁺和CD8⁺T细胞表面CTLA-4的表达,分析其与胶质瘤WHO分级及手术的关系。结果: 胶质瘤组患者外周血CD4⁺和CD8⁺T细胞表面CTLA-4的表达水平明显高于对照组(P<0.01);Ⅳ级胶质瘤患者CD4⁺和CD8⁺T细胞表面CTLA-4表达水平最高,Ⅳ级胶质瘤患者CD4⁺和CD8⁺T细胞表面CTLA-4表达水平高于Ⅲ级胶质瘤(P<0.01),Ⅲ级胶质瘤患者CD4⁺和CD8⁺T细胞表面CTLA-4表达水平高于Ⅱ级胶质瘤(P<0.01);术后胶质瘤患者CD4⁺和CD8⁺T细胞表面CTLA-4表达水平低于术前(P<0.01)。结论: 胶质瘤患者外周血CD4⁺和CD8⁺T细胞表面CTLA-4的表达水平随着胶质瘤恶性程度的升高而升高,并在胶质瘤发生发展过程中发挥促进作用。

关键词: 胶质瘤, T淋巴细胞, 细胞毒性T淋巴细胞相关抗原4

Abstract:

Objective: To investigate the correlation between the CTLA-4 expression in the peripheral blood CD4⁺ and CD8⁺ T cells in the patients with glioma and glioma WHO classification and operation, and to clarify the clinical significancesof CTLA-4.Methods: 60 patients with glioma from our hospital were selected as glioma group, and 46 healthy volunteers were used as control group.Then the CTLA-4 expression levels in the peripheral blood CD4⁺ and CD8⁺ T cells of the subjects in glioma group and control group were detected by fluorescence-activated cell sorting (FACS) and the relationship between CTLA-4 and glioma WHO classification and surgery was analyzed.Results: The CTLA-4 expression levels in CD4⁺ and CD8⁺ T cells in peripheral blood of the patients in glioma group were higher than those in control group(P<0.01).The CTLA-4 expression level in the patients with grade Ⅳ glioma was the highest, the expression level of the patients with grade Ⅳ glioma was higher than that of the patients with grade Ⅲ glioma(P<0.01), and the expression level of grade Ⅲ the patients with was higher than that of the patients with grade Ⅱ(P<0.01).The expression level of CTLA 4 in CD4⁺ and CD8⁺ T cells of the patients with glioma after operation was lower than beforeoperation(P<0.01).Conclusion: The CTLA-4 expression levels in the peripheral blood CD4⁺ and CD8⁺ T cells are increased with the increasing of malignancy degree of the patients with glioma, and it has a promotion role in the occurrence of development of glioma.

Key words: glioma, T lymphocyte, cytotoxic T-lymphocyte associated antigen-4

中图分类号:

R739.41

樊锐太, 张恒, 王鑫, 顾浩. 胶质瘤患者T淋巴细胞表面CTLA-4的表达及其临床意义[J]. 吉林大学学报(医学版), 2016, 42(02): 311-315.

FAN Ruitai, ZHANG Heng, WANG Xin, GU Hao. Expression of CTLA-4 in T lymphocytes of patients with glioma and its clinical significance[J]. Journal of Jilin University Medicine Edition, 2016, 42(02): 311-315.

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