子宫内膜癌组织中hMOF蛋白和mRNA表达及其临床意义

doi:10.13481/j.1671-587x.20160617

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (06): 1138-1142.doi: 10.13481/j.1671-587x.20160617

子宫内膜癌组织中hMOF蛋白和mRNA表达及其临床意义

文爱平¹, 汪海娇², 金景姬³, 蔡勇³, 朱继红⁴, 谌伦华¹

1. 川北医学院附属医院妇产科, 四川南充 637000;
2. 吉林大学第一医院妇产科, 吉林长春 130021;
3. 吉林大学生命科学院表观遗传学教研室艾滋病国家重点实验室, 吉林长春 130012;
4. 吉林大学第一医院生殖中心, 吉林长春 130021

收稿日期:2016-01-30 出版日期:2016-11-28 发布日期:2016-12-02
通讯作者: 朱继红,副教授,硕士研究生导师(Tel:0431-88782706,E-mail:zhu-ji-hong@sohu.com);谌伦华,副主任医师,硕士研究生导师(Tel:0817-2262078,E-mail:hua969@189.com) E-mail:zhu-ji-hong@sohu.com;hua969@189.com
作者简介:文爱平(1984-),男,四川省仪陇县人,主治医师,医学硕士,主要从事妇科肿瘤微创和表观遗传学方面的研究。
基金资助:
国家自然科学基金资助课题（31070668）

Expressions of hMOFprotein and mRNA in endometrial cancer tissue and their clinical significances

WEN Aiping¹, WANG Haijiao², JIN Jingji³, CAI Yong³, ZHU Jihong⁴, CHEN Lunhua¹

1. Departement of Obstetrics and Gynecology, Affiliated Hospital, North Sichuan Medical College, Nanchong 637000, China;
2. Department of Obstetrics and Gynecology, First Hospital, Jilin University, Changchun 130021, China;
3. National Key Laboratory of Acquired Immune Deficiency Syndrome, Department of Epigenetics, School of Life Sciences, Jilin University, Changchun 130012, China;
4. Reproductive Medicine Center, First Hospital, Jilin University, Changchun 30021, China

Received:2016-01-30 Online:2016-11-28 Published:2016-12-02

摘要/Abstract

摘要：

目的：探讨子宫内膜癌组织中hMOF蛋白和mRNA的表达，阐明其参与调控子宫内膜癌发生发展的可能机制。方法：采用RT-PCR和免疫组织化学染色方法检测38例子宫内膜腺癌组织及20例正常子宫内膜组织中hMOF蛋白和mRNA的表达，分析hMOF蛋白和mRNA表达水平与子宫内膜腺癌患者的临床病理特征和预后的关系。结果：免疫组织化学染色，子宫内膜腺癌组织中hMOF蛋白阳性表达率低于正常子宫内膜组织（P=0.037）；临床病理分期为Ⅰ期的患者hMOF蛋白阳性表达率高于Ⅱ期和Ⅲ-Ⅳ期患者（χ²=6.591，P=0.037）；子宫内膜癌低分化患者hMOF蛋白阳性表达率低于中分化和高分化患者（χ²=7.351，P=0.025）。RT-PCR检测，子宫内膜癌组织中hMOFmRNA表达上调者所占比率明显低于正常子宫内膜组织（P=0.003）。结论：hMOF蛋白可能参与了子宫内膜癌的发生发展过程，有望成为判断子宫内膜癌预后的生物学指标。

关键词: 乙酰基转移酶类, 腺癌, 聚合酶链反应, 免疫组织化学, 子宫内膜肿瘤

Abstract:

Objective: To discuss the expressions of hMOF protein and mRMA in endometrial cancer tissue,and to illuminate the possible mechanism of hMOF in the occurrence and development of endometrial cancer.Methods: The PT-PCR and immunohistochemistry methods were used to detect the expressions of hMOF protein and mRNA in 38 cases of endomerial cancer tissues and 20 cases of normal endometrial tissues,and to analyze the relationship between its expression level and the clinical characteristics and prognosis of the patients with endometrial cancer.Results: The immunohistochemistry results showed that the positive expression rate of hMOF protein in endometrial cancer tissue was lower than those in normal endometrial tissue(P=0.037); the positive expression rate of hMOF protein in stage Ⅰ patients was higher than those in stage Ⅱ and Ⅲ-Ⅳ patients(χ²=6.591,P=0.037);the positive exprssion rate of hMOF protein in the patients with poor differentiation was lower than those in the patients with moderate and high differentiation(χ²=7.351,P=0.025).The RT-PCR results showed that the percentage of the patients with expression up-regulation of hMOF mRNA in endometrial carcinoma tissue was remarkly decreased compared with normal endometrial tissue(P=0.003).Conclusion: hMOF protein may participate in the occurrence and development of endometrial cancer,and hMOF protein could be a biomarker for prognosis of endometrial cancer.

Key words: endometrial neoplasms, polymerase chain reaction, immunohistochemistry, acetyltransferases, adenocarcinoma

中图分类号:

R737.33

文爱平, 汪海娇, 金景姬, 蔡勇, 朱继红, 谌伦华. 子宫内膜癌组织中hMOF蛋白和mRNA表达及其临床意义[J]. 吉林大学学报(医学版), 2016, 42(06): 1138-1142.

WEN Aiping, WANG Haijiao, JIN Jingji, CAI Yong, ZHU Jihong, CHEN Lunhua. Expressions of hMOFprotein and mRNA in endometrial cancer tissue and their clinical significances[J]. Journal of Jilin University Medicine Edition, 2016, 42(06): 1138-1142.

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子宫内膜癌组织中hMOF蛋白和mRNA表达及其临床意义

Expressions of hMOFprotein and mRNA in endometrial cancer tissue and their clinical significances

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