多发性骨髓瘤和慢性淋巴细胞白血病患者血浆lncRNA MALAT1、LincRNA-p21和GAS5的表达水平及其诊断意义

doi:10.13481/j.1671-587x.20180224

吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (02): 339-345.doi: 10.13481/j.1671-587x.20180224

多发性骨髓瘤和慢性淋巴细胞白血病患者血浆lncRNA MALAT1、LincRNA-p21和GAS5的表达水平及其诊断意义

黄琦¹, 沙辉¹, 陈龙¹, 吕龙龙¹, 徐声鸣¹, 张泽天¹, 赵松¹, 靳凤艳², 牛丰¹

1. 吉林大学第一医院脊柱外科, 吉林长春 130021;
2. 吉林大学第一医院肿瘤中心, 吉林长春 130021

收稿日期:2017-12-05 出版日期:2018-03-28 发布日期:2018-03-30
通讯作者: 牛丰,副教授,硕士研究生导师(Tel:0431-81875658,E-mail:niufeng168@hotmail.com) E-mail:niufeng168@hotmail.com
作者简介:黄琦(1992-),男,河南省许昌市人,在读医学硕士,主要从事脊柱外科临床工作和多发性骨髓瘤基础方面的研究。
基金资助:
国家自然科学基金资助课题（81670190）

Expression levels of plasma lncRNA MALAT1, LincRNA-p21 and GAS5 in patients with multiple myeloma and chronic lymphocytic leukemia and their diagnostic significances

HUANG Qi¹, SHA Hui¹, CHEN Long¹, LYU Longlong¹, XU Shengming¹, ZHANG Zetian¹, ZHAO Song¹, JIN Fengyan², NIU Feng¹

1. Department of Spine Surgery, First Hospital, Jilin University, Changchun 130021, China;
2. Cancer Center, First Hospital, Jilin University, Changchun 130021, China

Received:2017-12-05 Online:2018-03-28 Published:2018-03-30

摘要/Abstract

摘要： 目的：探讨多发性骨髓瘤（MM）和慢性淋巴细胞白血病（CLL）患者血浆长链非编码RNA （lncRNA） MALAT1、LincRNA-p21和GAS5相对表达水平的差异，阐明3种lncRNA在MM与CLL鉴别诊断中的意义。方法：选取60例MM患者（MM组）、60例CLL患者（CLL组）和60名健康体检者（对照组）作为研究对象，对3组研究对象血浆lncRNA MALAT1、LincRNA-p21和GAS5的表达水平进行检测和比较。结果：MM组患者血浆lncRNA MALAT1和GAS5相对表达水平明显高于其他2组（P<0.05）。CLL组患者血浆LincRNA-p21相对表达水平明显低于其他2组（P<0.05）。血浆LincRNA-p21诊断CLL的受试者工作特征曲线（ROC）下面积（AUC）为0.850，95%可信区间（CI）为0.780~0.921；血浆lncRNA MALAT1和GAS5在MM诊断中的AUC分别为0.898和0.815，95% CI分别为0.836~0.959和0.740~0.890；血浆lncRNA MALAT1、LincRNA-p21和GAS5在CLL和MM鉴别诊断中的AUC分别为0.878、0.778和0.805，95% CI分别为0.814~0.942、0.691~0.865和0.727~0.882。结论：血浆lncRNA MALAT1和GAS5的高表达与MM的发病有关，而血浆LincRNA-p21低表达与CLL的发病有关，上述3种lncRNA的血浆相对表达水平可作为MM和CLL诊断中的辅助指标。

关键词: 血浆, 多发性骨髓瘤, 长链非编码RNA, 慢性淋巴细胞白血病

Abstract: Objective:To explore the differences of the relative expression levels of plasma long chain non encoding RNA (lncRNA) MALAT1, LincRNA-p21 and GAS5 in the patients with multiple myeloma (MM) and chronic lymphocytic leukemia (CLL), and to clarify their significances in the differential diagnosis of MM and CLL. Methods: A total of 60 cases of MM patients (MM group), 60 cases of CLL patients (CLL roup) and 60 healthy persons after physical examinations (control group) were selected as the subjects. The plasma levels of lncRNA MALAT, LincRNA-p21 and GAS5 of the subjects in three groups were detected and compared. Results: The relative expression levels of lncRNA MALAT1 and GAS5 in plasma of the patients in MM group were significantly higher than those in the other two groups(P<0.05);the relative expression level of LincRNA-p21 in plasma of the patients in CLL group was significantly lower than those in the other two groups(P<0.05).The area under receiver operating characteristic curve(ROC)(AUC) of plasma LincRNA-p21 in the diagnosis of CLL was 0.850, its 95% confidence interval (CI) was 0.780-0.921. The AUC of plasma lncRNA MALAT1 and GAS5 in the diagnosis of MM were 0.898 and 0.815; their 95% CI were 0.836-0.959 and 0.740-0.890, respectively. The AUC of plasma lncRNA MALAT1, LincRNA-p21 and GAS5 in the differential diagnosis of CLL and MM were 0.878, 0.778 and 0.805, and their 95%CI were 0.814-0.942, 0.691-0.865 and 0.727-0.882, respectively. Conclusion: The incidence of MM is related with the high expressions of lncRNA MALAT1 and GAS5 in plasma and the incidence of CLL is related with the low expression of LincRNA-p21 in plasma. The relative expression levels of the above lncRNA can be used as the auxiliary indexes in the diagnosis of MM and CLL.

Key words: chronic lymphocytic leukemia, plasma, long chain non encoding RNA, multiple myeloma

中图分类号:

R733.3

黄琦, 沙辉, 陈龙, 吕龙龙, 徐声鸣, 张泽天, 赵松, 靳凤艳, 牛丰. 多发性骨髓瘤和慢性淋巴细胞白血病患者血浆lncRNA MALAT1、LincRNA-p21和GAS5的表达水平及其诊断意义[J]. 吉林大学学报(医学版), 2018, 44(02): 339-345.

HUANG Qi, SHA Hui, CHEN Long, LYU Longlong, XU Shengming, ZHANG Zetian, ZHAO Song, JIN Fengyan, NIU Feng. Expression levels of plasma lncRNA MALAT1, LincRNA-p21 and GAS5 in patients with multiple myeloma and chronic lymphocytic leukemia and their diagnostic significances[J]. Journal of Jilin University Medicine Edition, 2018, 44(02): 339-345.

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多发性骨髓瘤和慢性淋巴细胞白血病患者血浆lncRNA MALAT1、LincRNA-p21和GAS5的表达水平及其诊断意义

Expression levels of plasma lncRNA MALAT1, LincRNA-p21 and GAS5 in patients with multiple myeloma and chronic lymphocytic leukemia and their diagnostic significances

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