miRNA-155在大鼠脑缺血/再灌注损伤早期大脑皮层组织中的表达及其意义

doi:10.13481/j.1671-587x.20180606

吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (06): 1144-1149.doi: 10.13481/j.1671-587x.20180606

miRNA-155在大鼠脑缺血/再灌注损伤早期大脑皮层组织中的表达及其意义

朱旭蓉^1,2, 燕玉娥¹, 狄政莉¹, 王天仲^1,2, 何芳¹, 王新来¹, 高晓宇^1,2, 郑雪娇^1,2

1. 陕西省西安市中心医院神经内科, 陕西西安 710003;
2. 延安大学医学院临床医学系, 陕西延安 716000

收稿日期:2018-02-06 出版日期:2018-11-28 发布日期:2018-11-28
通讯作者: 狄政莉,教授,硕士研究生导师(Tel:029-62664126,E-mail:zhenglidi@126.com) E-mail:zhenglidi@126.com
作者简介:朱旭蓉(1990-),女,陕西省延安市人,医学硕士,主要从事缺血性脑卒中基础方面的研究。
基金资助:
国家自然科学基金资助课题（8150051170）；陕西省中医药管理局科研项目资助课题（JCMS065）

Expression of miRNA-155 in cerebral cortex tissue of rats with cerebral ischemia-reperfusion injury and its significance

ZHU Xurong^1,2, YAN Yue¹, DI Zhengli¹, WANG Tianzhong^1,2, HE Fang¹, WANG Xinlai¹, GAO Xiaoyu^1,2, ZHENG Xuejiao^1,2

1. Department of Neurology, Central Hospital, Xi'an City, Shannxi Province, Xi'an 710003, China;
2. Depentment of Clinical Medicine, School of Medical Sciences, Yan'an University, Yan'an 716000, China

Received:2018-02-06 Online:2018-11-28 Published:2018-11-28

摘要/Abstract

摘要： 目的：探讨微小RNA-155（miRNA-155）在大鼠脑缺血/再灌注损伤早期大脑皮层组织中的表达，初步阐明miRNA-155对脑缺血/再灌注损伤的影响。方法：48只健康成年雄性SD大鼠，随机分为假手术组和脑缺血/再灌注组，每组24只。脑缺血/再灌注组通过Longa等改良线栓法栓塞大鼠右侧大脑中动脉建立脑缺血/再灌注模型，假手术组大鼠只分离血管。采用TTC染色评估各组大鼠脑缺血梗死体积，采用qRT-PCR法检测各组大鼠脑皮层组织中miRNA-155表达水平。结果：与假手术组比较，再灌注24、48和72h时脑缺血/再灌注组大鼠脑缺血梗死体积明显增大（P<0.05），并且随着再灌注时间延长，脑缺血梗死体积逐渐减少。与假手术组比较，再灌注24、48和72 h时脑缺血/再灌注组大鼠脑皮层组织中miRNA-155表达水平明显升高（P<0.05），并且随着灌注时间延长，表达水平逐渐降低。结论：在大鼠脑缺血/再灌注早期大脑皮层组织中miRNA-155高表达，其参与了脑缺血/再灌注损伤过程。

关键词: 微小RNA-155, 脑缺血, 再灌注损伤, 大鼠,SD

Abstract: Objective: To investigate the expression of microRNA-155(miRNA-155) in the cerebral cortex tissue of the rats with cerebral ischemia-reperfusion injury, and to clarify the effect of miRNA-155 on cerebral ischemia-reperfusion injury.Methods: A total of 48 healthy adult male SD rats were randomly divided into sham operation group(n=24) and cerebral ischemia reperfusion group(I/R group,n=24).The cerebral ischemia-reperfusion models in I/R group were established by Longa modified suture occlusion in the right middle cerebral artery of the rats.The rats in sham operation group only received blood vessel isolation.TTC staining was used to calculate the ischemic infarction volume of the rats in various groups.qRT-PCR method was used to detect the expression levels of miR-155 in cerebral cortex tissue of the rats in various groups.Results: Compared with sham operation group, the ischemic infarction volume of the rats in I/R group was significantly increased at 24,48,and 72 h after reperfusion(P<0.05);the ischemic infarction volumes of the rats in I/R group were decreased gradually with the prolongation of reperfusion time.Compared with sham operation group,the expression levels of miR-155 in cerebral cortex tissue of the rats in I/R group were significantly increased at 24, 48, and 72 h after reperfusion (P<0.05); the expression levels of miR-155 were gradually decreased with the prolongation of reperfusion time.Conclusion: The expression levels of miR-155 in cerebral cortex tissue of the rats with cerebral ischemia-reperfusion injury are high, and miR-155 may participate in the process of cerebral ischemia-reperfusion injury.

Key words: microRNA-155, cerebral ischemia, reperfusion injury, rats,SD

中图分类号:

R651.15

朱旭蓉, 燕玉娥, 狄政莉, 王天仲, 何芳, 王新来, 高晓宇, 郑雪娇. miRNA-155在大鼠脑缺血/再灌注损伤早期大脑皮层组织中的表达及其意义[J]. 吉林大学学报(医学版), 2018, 44(06): 1144-1149.

ZHU Xurong, YAN Yue, DI Zhengli, WANG Tianzhong, HE Fang, WANG Xinlai, GAO Xiaoyu, ZHENG Xuejiao. Expression of miRNA-155 in cerebral cortex tissue of rats with cerebral ischemia-reperfusion injury and its significance[J]. Journal of Jilin University(Medicine Edition), 2018, 44(06): 1144-1149.

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miRNA-155在大鼠脑缺血/再灌注损伤早期大脑皮层组织中的表达及其意义

Expression of miRNA-155 in cerebral cortex tissue of rats with cerebral ischemia-reperfusion injury and its significance

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