茯苓复合提取物对2型糖尿病大鼠血糖和血脂水平的影响

doi:10.13481/j.1671-587x.20200507

摘要/Abstract

摘要： 目的：探讨茯苓复合提取物对2型糖尿病（T2DM）大鼠血糖、血脂水平和抗氧化能力的影响，并阐明其作用机制。方法：将65只SPF级SD雄性大鼠适应性喂养1周后，随机选取10只为对照组，其余大鼠采用高糖高脂喂养联合腹腔注射链脲佐菌素（STZ）30 mg·kg^-1建立T2DM大鼠模型，造模成功的大鼠随机分为模型组，二甲双胍组，低、中和高剂量茯苓复合提取物组。低、中和高剂量茯苓复合提取物组大鼠分别给予1.5、3.0和6.0 g·kg^-1茯苓复合提取物，对照组和模型组大鼠给予等体积双蒸水，连续4周。检测各组大鼠体质量和空腹血糖（FBG）水平，检测各组大鼠血清中胰岛素（INS）、胰高血糖素（GC）、甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）和丙二醛（MDA）水平及超氧化物歧化酶（SOD）活性，采用稳态模型评估胰岛素抵抗指数（HOMA-IR）。结果：与对照组比较，模型组大鼠体质量明显降低（P<0.05），FBG水平明显升高（P<0.05），血清中GC、TG、TC、LDL-C和MDA水平及HOMA-IR均明显升高（P<0.05），血清中INS和HDL-C水平及SOD活性均明显降低（P<0.05）。与模型组比较，低、中和高剂量茯苓复合提取物组大鼠体质量均明显升高（P<0.05），FBG水平均明显降低（P<0.05），血清中GC、TG、TC、LDL-C和MDA水平及HOMA-IR均明显降低（P<0.05），血清中INS和HDL-C水平及SOD活性均明显升高（P<0.05）。结论：茯苓复合提取物具有降低T2DM大鼠FBG水平、调节脂代谢紊乱和抗氧化的作用，并可明显改善胰岛素抵抗。

关键词: 茯苓复合提取物, 链脲佐菌素, 空腹血糖, 脂代谢, 抗氧化, 胰岛素抵抗

Abstract: Objective: To study the effects of poria cocos complex extract on the levels of blood glucose and blood lipid and the antioxidant capacity in the rats with type 2 diabetes mellitus(T2DM), and to elucidate its mechanism. Methods: After 65 male SPF SD rats were adaptively fed for 1 week,10 rats were randomly selected as control group; the rest of rats were fed with high glucose and fat and intraperitoneally injected with STZ (30 mg·kg^-1) to establish the T2DM rat models. The model rats were randomly divided into model group, metformin group,and low, and medium and high doses of poria cocos compound extract groups. The rats in low, medium and high doses of poria cocos compound extract groups were administered with different doses (1.5, 3.0 and 6.0 g·kg^-1) of poria cocos compound extract by gavage for 4 weeks, and the rats in control group and model group were given the same volume of double distilled water for 4 weeks. The body weights and the levels of fasting blood glucose (FBG) of the rats in various groups were detected, the levels of serum insulin (INS), glucagon (GC), triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) of the rats in various groups were measured, and the homeostasis model was used to evaluate the insulin resistance index (HOMA-IR). Results: Compared with control group, the body weight of the rats in model group was significantly reduced(P<0.05), the FBG level was significantly increased (P<0.05), the serum GC, TG, TC, LDL-C and MDA levels and HOMA-IR were significantly increased(P<0.05), and the serum INS and HDL-C levels and the SOD activity were significantly decreased(P<0.05). Compared with model group, the body weights of the rats in low, medium and high doses of poria cocos compound extract groups were significantly increased(P<0.05), the FBG levels were significantly decreased(P<0.05), the serum GC, TG, TC, LDL-C and MDA levels and HOMA-IR were significantly decreased(P<0.05),and the serum INS and HDL-C levels and the SOD activities were significantly increased(P<0.05). Conclusion: Poria cocos compound extract can reduce the level of FBG, regulate lipid metabolism disorder and resist the oxidation in the T2DM rats, and significantly improve insulin resistance.

Key words: poria cocos compound extract, streptozotocin, fasting blood glucose, lipid metabolism, antioxidant, insulin resistance

中图分类号:

R587.1

张叶茁, 杨岳峰, 杨野仝, 张颖, 刘雅娟. 茯苓复合提取物对2型糖尿病大鼠血糖和血脂水平的影响[J]. 吉林大学学报(医学版), 2020, 46(05): 937-941.

ZHANG Yezhuo, YANG Yuefeng, YANG Yetong, ZHANG Ying, LIU Yajuan. Effects of poria cocos compound extract on blood glucose and lipid levels in rats with type 2 diabetes mellitus[J]. Journal of Jilin University(Medicine Edition), 2020, 46(05): 937-941.

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