吉林大学学报(医学版) ›› 2022, Vol. 48 ›› Issue (3): 615-621.doi: 10.13481/j.1671-587X.20220309

• 基础研究 • 上一篇    下一篇

新型nHA/PLGA纳米复合微球对大鼠颅骨缺损的修复作用

潘洁1,汪德州2,宋文植2()   

  1. 1.长春医学高等专科学校口腔教研室, 吉林 长春 130031
    2.吉林大学中日联谊医院口腔科, 吉林 长春 130021
  • 收稿日期:2021-08-18 出版日期:2022-05-28 发布日期:2022-06-21
  • 通讯作者: 宋文植 E-mail:343904478@qq.com
  • 作者简介:潘 洁(1986-),女,吉林省长春市人,主治医师,医学硕士,主要从事口腔修复学相关方面的研究。
  • 基金资助:
    吉林省科技厅重点研发项目(20210204111YY)

Repair effect of new nHA/PLGA nanocomposite microspheres on skull defects of rats

Jie PAN1,Dezhou WANG2,Wenzhi SONG2()   

  1. 1.Department of Stomatology, Changchun Medical College, Changchun 130031, China
    2.Department of Stomatology, China-Japan Uonin Hospital, Jilin University, Changchun 130021, China
  • Received:2021-08-18 Online:2022-05-28 Published:2022-06-21
  • Contact: Wenzhi SONG E-mail:343904478@qq.com

摘要: 目的

探讨新型纳米羟基磷灰石(nHA)/聚丙交酯-乙交酯(PLGA)纳米复合微球对大鼠颅骨缺损的修复作用,为该材料的临床应用提供依据。

方法

采用口腔低速手机于9只SD大鼠的颅骨制备2个对称的5 mm直径临界骨缺损模型,并随机分为实验组(植入nHA/PLGA纳米复合微球)、对照组(植入Bio-oss颗粒)和空白组(仅制备缺损而未填充材料),每组6个颅骨缺损模型。于术后当天、术后4周和术后8周进行颅骨CT三维重建,检查颅骨缺损修复效果;于第8周处死大鼠,采用微计算机断层扫描技术(micro-CT)扫描三维重建,观察各组大鼠颅骨缺损区域骨小梁数量(Tb.N)、缺损区域骨小梁间距(Tb.Sp)、缺损区域骨小梁厚度(Tb.Th)和缺损区域新生骨量与缺损总体积的比值(BV/TV)。

结果

肉眼观察,实验组大鼠颅骨缺损区域边界清晰,颗粒间隙内可见新生骨质;对照组大鼠颅骨缺损区域见颗粒状骨粉堆积,无明显吸收;空白组大鼠颅骨缺损中心区域有明显凹陷,无明显骨沉积。CT三维重建检查,在第8周,与空白组比较,实验组大鼠有明显的新骨形成;与对照组比较,实验组大鼠均出现明显的新骨沉积。micro-CT检查,在第8周,与对照组比较,实验组大鼠颅骨缺损区Tb.N、Tb.Sp和Tb.Th差异无明显统计学意义(P>0.05),BV/TV降低(P<0.05);与空白组比较,实验组和对照组大鼠颅骨缺损区Tb.Sp、Tb.Th和BV/TV比较差异均有统计学意义(P<0.05)。

结论

nHA/PLGA纳米复合微球对骨缺损具有良好的修复效果。

关键词: 颅骨缺损, 骨修复, Bio-Oss颗粒, 骨组织工程, 纳米羟基磷灰石/聚丙交酯-乙交酯纳米复合微球

Abstract: Objective

To investigate the repair effect of new nano-hydroxyapatite (nHA)/polylactide-glycolide (PLGA) nanocomposite microspheres on the skull bone defects of the rats, and to provide the basis for the clinical application of the material.

Methods

Two symmetrical critical bone defect models with a diameter of 5 mm were prepared in the skulls of 9 SD rats with a dental low-speed handpiece, and they were randomly divided into experimental group (implanted with nHA/PLGA nanocomposite microspheres), control group (implanted with Bio-Oss particles) and blank group (only the defect was prepared without filling material),and there were 6 skull defect models in each group. The CT three dimensional reconstruction of skull was performed on the right postoperative day, 4 weeks after surgery and 8 weeks after surgery to check the repair effect of skull defect.The rats were sacrificed at the 8th week,and micro-computed tomography (micro-CT) scanning was used to scan the three-dimensional reconstruction, and the number of trabecular bone(Tb.N ), trabecular bone spacing (Tb.Sp), trabecular bone thickness (Tb.Th), and the ratios of new bone volume/total bone volume (BV/TV)in skull defect area of the rats in various groups were detected.

Results

The naked eye observation results showed that the boundary of the skull defect area of the rats in experimental group was clear, and new bone could be seen in the intergranular space; in control group, there was granular bone powder accumulation in the skull defect area without obvious absorption; in blank group, there was obvious depression in the central area of skull defect without obvious bone deposition.The CT three-dimensional reconstruction results showed that 8 weeks after sugery,compared with blank group, the rats in experimental group had obvious new bone formation; compared with control group, the rats in experimental group had obvious new bone deposition.The micro-CT detection results showed that 8 weeks after sugery, compared with control group, there were no significant differences in the Tb.N, Tb.Sp and Tb.Th in skull defect area of the rats in experimental group (P>0.05), and the BV/TV was decreased (P<0.05); compared with blank group, there were significant differences in the Tb.Sp, Tb.Th and BV/TV in skull defect area of the rats in experimental group and control group (P<0.05).

Conclusion

nHA/PLGA nanocomposite microspheres have a good repair effect on the bone defects.

Key words: Skull defect, Bone repair, Bio-Oss particles, Bone tissue engineering, Nano-hydroxyapatite/polylactide-glycolide nanocomposite microspheres

中图分类号: 

  • R782