关键词: 实验性, 并发症, 内耳病变, 鱼油, 治疗应用, 诱发电位, 听觉, 脑干

Abstract: Objective To observe the preventive effect of combination fish oil on pathological lesions in inner ear of diabetic rats and its mechanism. Methods Fifty male Wistar rats were randomly divided into three groups randomly :group A (normal control, n=16), group B (diabetic control, n=18), and group C (treatment group,n=16). Rats in group B and group C were injected intravenously with streptozocin(STZ) of 55 mg•kg^-1. Three weeks after injection,the rats in group C were continuously fed on combination fish oil everyday for 9 weeks. The levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C) and very low density lipoprotein-cholesterol (VLDL-C) in blood of rats were detected.The animal auditory brain stem evoked potentials were recorded. Results The levels of TC,TG,LDL, and VLDL in group C descented obviously compared with group B (P<0.05), auditory brain stem evoked potentials wave Ⅲ was obviously shortened in latent period compared with group B (P<0.05) and the threshold of wave Ⅲ decreased (P<0.05). Mass of lipid droplet in hairy cells in inner ear, vacuolar degeneration of mitochondrion and Golgi′s apparatus, hypertrophy and hyperplasia of endothelial cells in micrangium, and thick basement membrane were observed under transmission electron microscopy in group B. But these pathological lesions did not appear in the rats in group C. Conclusion Combination fish oil can prevent the lesions of inner ear in diabetic rats.

Key words: experimental, complication, lesions of inner ear, fish oils, therapeutic use, evoked potentials, auditory, brain stem