损伤肝组织匀浆诱导间充质干细胞甲胎蛋白和白蛋白的表达

doi:吉林省科技厅国际合作项目资助课题(2007072

损伤肝组织匀浆诱导间充质干细胞甲胎蛋白和白蛋白的表达

赵文静^1,2，方艳秋¹，齐亚灵¹，刘婷^*，牛凤兰²，谭岩¹

(1.吉林大学第一医院中心实验室,吉林长春 130021;2.吉林大学公共卫生学院卫生检验教研室,吉林长春 130021)

收稿日期:2008-06-10 修回日期:1900-01-01 出版日期:2008-11-28 发布日期:2008-11-28
通讯作者: 方艳秋

Expression of AFP and Alb in MSCs induced by injured liver tissue homogerate

ZHAO Wen-jing^1,2,FANG Yan-qiu¹,QI Ya-ling¹,LIU Ting^*,NIU Feng-lan²,TAN Yan¹

1.Central Laboratary，First Hospital,Jilin Univerdity ，Changchun 130021,China;2. Department of Health Inspection，School of Public Health，Jilin University， Changchun 130021, China)

Received:2008-06-10 Revised:1900-01-01 Online:2008-11-28 Published:2008-11-28
Contact: FANG Yan-qiu

摘要/Abstract

摘要： 目的：建立分离、纯化大鼠骨髓间充质干细胞（MSCs）的方法，探讨损伤肝组织匀浆对MSCs的诱导分化作用。方法：采用密度梯度离心、贴壁培养法分离培养大鼠MSCs，以含10%损伤肝组织匀浆、10%FBS的L-DMEM培养基诱导培养，并在不同时间点采用免疫组织化学方法（SP）检测甲胎蛋白（AFP）和白蛋白（Alb）的表达。结果：MSCs在含10%损伤肝组织匀浆的10%FBS L-DMEM培养基中培养，呈类上皮样细胞。诱导组在培养7 d时，大部分细胞呈AFP阳性表达（75.2%），与对照组比较差异有显著性(P<0.05)；诱导培养 14、21和28 d AFP表达阳性率分别为35.2%、12.3%和2.0%，组间比较差异有显著性（P<0.05）；诱导组在培养7 d仅少数细胞Alb呈阳性表达（14.6%），诱导培养14、21和28 d Alb表达阳性率分别为67.6%、88.9%和95.6%，诱导组与对照组比较及不同时间点比较差异均有显著性(P<0.05)。结论：此方法可获得纯度较高MSCs，损伤肝组织匀浆可诱导MSCs表达AFP和Alb。

关键词: 甲胎蛋白, 白蛋白

Abstract: To set up the method to isolate and purify rat bone marrow mesenchymal stem cells(MSCs) and to investigate the inducing effect of injured liver tissue homogenate.Methods MSCs were isolated and purified from bone marrow of young rat by density gradient centrifugation and adherent culture.MSCs were induced in L-DMEM with 10% injured liver tissue homogenate and 10%FBS in vitro.The expressions of alpha-fetoprotein(AFP) and albumin(Alb) were detected by immunohistochemistry at different time.Results MSCs became epithelia-shaped cells after cultivated in L-DMEM with 10% injured liver tissue homogenate and 10%FBS in vitro. Most cells were AFP positive after cultivated for 7 d(75.2%),compared with control group there was significant difference( P<0.05); AFP positive rates were respectively 35.2%,12.3% and 2.0% after cultivated for 14,21 and 28 d,there were significant differences between various groups(P<0.05); Only a small number of cells were Alb positive after cultivated for 7 d(14.6%).Alb positive rates were respectively 67.6%,88.9% and 95.6% after cultivated for 14,21 and 28 d,compared with control group and between different time points there were significant differences (P<0.05). Conclusion A method for isolation and purification of MSCs from bone marrow in vitro has been established.Injured liver tissue homogenate could induce the ex pressions of AFP and Alb in MSCs.

Key words: alpha-fetoproteins, albumins

中图分类号:

R329.2

赵文静，方艳秋，齐亚灵，刘婷，牛凤兰，谭岩. 损伤肝组织匀浆诱导间充质干细胞甲胎蛋白和白蛋白的表达[J]. J4, 2008, 34(6): 971-974.

ZHAO Wen-jing,FANG Yan-qiu,QI Ya-ling,LIU Ting,NIU Feng-lan,TAN Yan. Expression of AFP and Alb in MSCs induced by injured liver tissue homogerate[J]. J4, 2008, 34(6): 971-974.

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