关键词: 重组卡介苗, 结核分支杆菌

Abstract: Objective To construct phosphate-specific transport system 1 (PstS1)-BCG recombinant vaccine. Methods PstS1 gene was amplified from the genome of mycobacterium tuberculosis H37Rv and was cloned into E.coli - M.bovis BCG shuttle plasmid PMD31 to get recombinant plasmid S-PMD31. The S-PMD31 was introduced into E. coli and PstS1 expressed in high level induced by IPTG. S-PMD31 was then introduced into BCG by transformation or electroporation. Results 1 100 bp PstS1 gene was amplified from the genome of mycobacterium tuberculosis H37Rv, and then was cloned into E.coli - M. Bovis BCG shuttle plasmid PMD31. The PstS1 gene was correcctly inserted into shuttle plasmid PMD31 confirmed by restriction endonuclease digestion analyzing and DNA sequencing. PstS1 protein could be expressed in E.coli and then was introduced into M.bovis BCG by electroporation. Conclusion The recombinant PstS1-BCG vaccine is successfully constructed. It is anticipate that the PstS1-BCG vaccine can improve the immunogenicity and protective efficacy of BCG vaccine.

Key words: recombinant BCG, mycobacterium tuberculosis