J4

• 基础研究 • 上一篇    下一篇

低剂量辐射对裸鼠移植人胶质瘤(U251)细胞凋亡相关基因mRNA表达的影响

姜宏宇1，张国成1，王冠军2*   

  1. 1. 吉林大学第一医院老年病科，吉林 长春130021; 2. 吉林大学第一医院血液肿瘤科，吉林 长春130021
  • 收稿日期:2005-04-30 修回日期:1900-01-01 出版日期:2006-03-28 发布日期:2006-03-28
  • 通讯作者: 王冠军2*

Effect of low dose radiation on expression of apoptosis-related gene mRNA of transplantation tumor tissues of humanglioma (U251)-bearing nude mice

JIANG Hong-yu1, ZHANG Guo-cheng1，WANG Guan-jun2*   

  1. 1. Department of Gerontology, First Hospital, Jilin University, Changchun 130021,China; 2. Department of Hematology-Oncology,First Hospital, Jilin University, Changchun 130021,China
  • Received:2005-04-30 Revised:1900-01-01 Online:2006-03-28 Published:2006-03-28
  • Contact: WANG Guan-jun2*

RichHTML

0

赞

摘要： 目的：研究低剂量辐射对裸鼠移植人胶质瘤U251细胞凋亡相关基因mRNA表达的影响。方法：35只荷人胶质瘤U251瘤裸小鼠，随机分为7个实验组，假照组：0 mGy；D1组：75 mGy；D2组：4 Gy；D1-12 h+D2组：D1照射后12 h予以D2；D1-24 h+D2组：D1照射后24 h予以D2；D1-48 h+D2组：D1照射后48 h时予以D2；D1-72 h+D2组：D1照射后72 h予以D2。每组5只。各实验组不同方式照射后4 d，荷瘤裸鼠全部处死，采用原位杂交技术检测荷瘤组织的p53、Bcl-2 和Bax的mRNA表达水平。结果：D1（75 mGy）和D2（4 Gy）照射后，胶质瘤细胞的p53和Bax的mRNA表达分别呈上升趋势，Bcl-2的mRNA表达呈下降趋势，但与假照组比较，差异无显著性（P＞0.05）； D1+D2组（D1和D2间隔24、48及72 h）的p53和Bax的mRNA表达明显增多，Bcl-2 mRNA表达显著下降，与假照组比较差异有显著性（P＜0.05或P＜0.01）。结论：低剂量辐射在一定程度上可能上调了胶质瘤细胞的p53、Bax的mRNA表达，下调了Bcl-2 mRNA的表达，同时对其后的大剂量辐射有协同作用。

关键词: 人胶质瘤（U251）, 裸小鼠, 细胞凋亡, 基因, 原位杂交

Abstract: Objective To study the effects of low dose radiation（LDR）on expression of apoptosis-related gene mRNA of transplantation tumor tissues of human glioma (U251)-bearing nude mice.Methods Thirty -five tumor-bearing nude mice were divided into seven groups: sham-irradiated group：0 mGy；D1 group：75 mGy；D2 group：4 Gy；D1-12 h+D2 group：between D1 and D2 was 12 h; D1-24 h+D2 group：between D1 and D2 was 24 h；D1-48 h+D2 group：between D1 and D2 was 48 h；D1-72 h+D2 group：between D1 and D2 was 72 h. The expression of apoptosis-related gene mRNA of transplantation tumor tissues of tumor(U251)- bearing nude mice was detected by in situ hybridization. Results There were basal expressions of p53, Bcl-2, Bax mRNA in all groups. The transcription levels of p53 and Bax were slightly up-regulated and Bcl-2 was slightly down-regulated in D1（75 mGy）group and D2（4 Gy） group, but there was no significant difference compared with sham-irradiated group (0 mGy)（P>0.05）; While the differences of the levels of p53, Bcl-2, Bax between all D1+D2 groups and sham-irradiated group were significant (P＜0.05 or P＜0.01). Conclusion LDR could up-regulate the transcription level of p53, Bax and down-regulate Bcl-2 to some extent, and it maybe have synergic effect with high dose radiation.

Key words: human glioma (U251), nude mice, apoptosis, gene, in situ hybridization

版权所有 © 《吉林大学学报(医学版)》编辑部
地址：长春市新民大街828号(130021)　电话：+86-431-85619279　E-mail: xuebao@jlu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发