关键词: 肾阳虚证；心肌梗死；疾病模型, 动物；大鼠, Wistar

Abstract:

Objective
 To construct the myocardial infarction models in rats with kidney-yang deficiency and study the differences in myocardial morphology,myocardial enzymology and hemorheology between the myocardial infarction models with kidney-yang deficiency and the simple myocardial infarction models in rats and to provide evidence for evaluation of pharmacodynamics of medicine for curing chest and heart pain.Methods  60 Wistar rats were randomly divided into control group,kidney-yang deficiency models group,myocardial infarction sham operation group,myocardial infarction models group and myocardial infarction models with kidney-yang deficiency group,and there were 12 rats in each group.The myocardial infarction models under the status of the kidney-yang deficiency in rats were set up to determine the parameters of myocardial infarct size (MIS) and the activities of serum aspartate aminotransferase (AST),creatine phosphokinase (CK) and lactate dehydrogenase (LDH),at the same time,the platelet agglutinate rate (PAR),platelet aggregation(PAG),erythrocyte sedimentation rate(ESR),hematocrit(HCT) and the length,the dry weight,the wet weight of the thromb in vitro were tested,the parameters of thrombelastogram were also be detected.Results There were no obvious differences in MIS,the activities of CK,AST and LDH,and PAR,PAG,ESR and HCT between the myocardial infarction models with kidney-yang deficiency in rats and the simple myocardial infarction models（P>0.05）;the dry weight of the thromb and the length of thromb in vitro of the myocardial infarction models with kidney-yang deficiency in rats were heavier than those of the simple myocardial infarction models and the kidney-yang deficiency models in rats,but there were no significant differences（P>0.05）.The increasing extent of the values of r,k and the reducing extent of the value of ma in the myocardial infarction models with kidney-yang deficiency in rats were higher than those in the simple myocardial infarction models,but there were no significant differences between them（P>0.05）.Conclusion The obvious differences are not showed in the parameters of the indexes of MIS,the myocardium enzyme in the serum,HCT and ESR,the indexes of the function of the blood plate,the weight of the thromb in vitro and the thrombelastogram.

Key words:  kidney-yang deficiency;myocardial infarction;disease model,animal;rat,Wistar